Wang Bin, Wang Bin, Liu Peixin, Li Tao, Si Wei, Xiu Jinsheng, Liu Henggui
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, China; College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, China.
PLoS One. 2014 Jun 17;9(6):e99905. doi: 10.1371/journal.pone.0099905. eCollection 2014.
Newcastle disease virus (NDV) is a member of the Paramyxovirinae subfamily and can infect most species of birds. It has been a great threat for the poultry industry all around the world. In this report, we successfully produced infectious pseudotyped pNL4-3-Luc-R(-)E(-) (HIV-Luc) viruses with the HN and F envelope proteins of NDV. Further investigation revealed the cytoplasmic domains of HN and F, especially HN, plays a significant role in the infection efficiency of these pseudotyped HIV-Luc viruses. Replacement of, or direct fusion to the cytoplasmic domain of the HN protein by that of vesicular stomatitis virus G (VSV-G) could greatly enhance or destroy the infective potential of HN and F-pseudotyped (NDV-pseudotyped) HIV-Luc virus. We further established a novel neutralization assay to evaluate neutralizing antibodies against NDV with the NDV-pseudotyped HIV-Luc viruses. Comparative neutralization data indicate that the results determined by using the NDV-pseudotyped HIV-Luc viruses are as reliable as those by the conventional virus-neutralization assay (VN test) with native NDV. Moreover, the results show that the novel neutralization assay is more sensitive than the VN test.
新城疫病毒（NDV）是副粘病毒亚科的成员，可感染大多数鸟类物种。它一直对全球家禽业构成巨大威胁。在本报告中，我们成功地用NDV的血凝素神经氨酸酶（HN）和融合蛋白（F）包膜蛋白产生了感染性假型pNL4-3-Luc-R(-)E(-)（HIV-Luc）病毒。进一步研究表明，HN和F的细胞质结构域，尤其是HN，在这些假型HIV-Luc病毒的感染效率中起重要作用。用水泡性口炎病毒G（VSV-G）的细胞质结构域替换HN蛋白的细胞质结构域或直接与之融合，可极大地增强或破坏HN和F假型（NDV假型）HIV-Luc病毒的感染潜力。我们进一步建立了一种新型中和试验，以用NDV假型HIV-Luc病毒评估针对NDV的中和抗体。比较中和数据表明，使用NDV假型HIV-Luc病毒确定的结果与使用天然NDV的传统病毒中和试验（VN试验）的结果一样可靠。此外，结果表明新型中和试验比VN试验更敏感。
