Department of Animal Sciences, The Ohio State University, Columbus, OH, USA.
Poult Sci. 2012 Jul;91(7):1654-9. doi: 10.3382/ps.2011-02029.
The selection processes that have resulted in broiler (meat) and leghorn (eggs) chickens have had very different effects on the pectoralis major and supracoracoideus muscles. The objective of this study, therefore, was to analyze the one-dimensional proteomic profiles of sarcoplasmic protein fractions isolated from the p. major and supracoracoideus muscles collected from 10 chicks from each genotype to compare developmental differences. The sarcoplasmic protein fraction was analyzed by SDS-PAGE. The mean band percentages were analyzed using a mixed model, with strain and muscle type as main effects. Six bands were found to be significantly different across the 2 strains. Strain differences in glycogen phosphorylase, enolase, elongation factor 1, creatine kinase, fructose-bisphosphate aldolase, and glyceraldehyde 3-phosphate-dehydrogenase suggest a genotype-specific shift in energy metabolism during breast muscle growth and development.
选择过程导致肉鸡（肉用）和来航鸡（蛋用）在胸大肌和上胸软骨肌上产生了非常不同的影响。因此，本研究的目的是分析从每个基因型的 10 只小鸡中采集的胸大肌和上胸软骨肌的肌浆蛋白分离的一维蛋白质组图谱，以比较发育差异。肌浆蛋白部分通过 SDS-PAGE 进行分析。使用混合模型分析平均带百分比，以品系和肌肉类型为主要效应。在 2 个品系中发现 6 个条带存在显著差异。糖原磷酸化酶、烯醇酶、延伸因子 1、肌酸激酶、果糖二磷酸醛缩酶和甘油醛-3-磷酸脱氢酶在 2 个品系中的差异表明，在胸肌生长和发育过程中，能量代谢存在特定的基因型变化。
