煤尘提取物诱导BALB/3T3 A31-1-13细胞系发生形态转化。

Induction of morphological transformation by coal-dust extract in BALB/3T3 A31-1-13 cell line.

作者信息

Wu Z L, Chen J K, Ong T, Matthews E J, Whong W Z

机构信息

Division of Respiratory Disease Studies, National Institute for Occupational Safety and Health, Morgantown, WV 26505-2888.

出版信息

Mutat Res. 1990 Nov;242(3):225-30. doi: 10.1016/0165-1218(90)90088-j.

DOI:10.1016/0165-1218(90)90088-j

PMID:2270094

Abstract

The transforming activity of coal dust extracts was studied using BALB/3T3 clone A31-1-13 cells. Coal-dust extracts, both nitrosated and nonnitrosated, induced cell transformation in a dose-response manner. However, the transformation frequency was higher with the nitrosated than with the nonnitrosated extract. All transformed cell lines derived from coal-dust extract-induced foci showed biological characteristics of neoplastic transformation such as loss of contact inhibition and anchorage-independent growth. These results appear to support a hypothesis of coal mine dust causation of gastric cancer in coal miners.

摘要

使用BALB/3T3克隆A31-1-13细胞研究了煤尘提取物的转化活性。无论是亚硝化的还是未亚硝化的煤尘提取物，均以剂量反应方式诱导细胞转化。然而，亚硝化提取物的转化频率高于未亚硝化提取物。所有源自煤尘提取物诱导灶的转化细胞系均表现出肿瘤转化的生物学特征，如接触抑制丧失和不依赖贴壁生长。这些结果似乎支持了煤矿粉尘导致煤矿工人患胃癌的假说。