Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland 4072, Australia.
J Biol Chem. 2012 Aug 3;287(32):26999-7000. doi: 10.1074/jbc.R112.390344. Epub 2012 Jun 14.
Circular proteins have now been discovered in all kingdoms of life and are characterized by their exceptional stability and the diversity of their biological activities, primarily in the realm of host defense functions. This thematic minireview series provides an overview of the distribution, evolution, activities, and biological synthesis of circular proteins. It also reviews approaches that biological chemists are taking to develop synthetic methods for making circular proteins in the laboratory. These approaches include solid-phase peptide synthesis based on an adaption of native chemical ligation technology and recombinant DNA approaches that are amenable to the in-cell production of cyclic peptide libraries. The thioester-mediated native chemical ligation approach mimics, to some extent, elements of the natural biosynthetic reaction, which, for disulfide-rich cyclic peptides, appears to involve asparaginyl endopeptidase-mediated processing from larger precursor proteins.
现已在所有生命领域发现了环状蛋白，其特征是具有异常的稳定性和生物活性的多样性，主要存在于宿主防御功能领域。本专题综述系列概述了环状蛋白的分布、进化、活性和生物合成。它还回顾了生物化学家正在采取的方法，以开发在实验室中制造环状蛋白的合成方法。这些方法包括基于天然化学连接技术改编的固相肽合成和适用于环状肽文库的细胞内生产的重组 DNA 方法。硫酯介导的天然化学连接方法在某种程度上模拟了天然生物合成反应的元素，对于富含二硫键的环状肽，该反应似乎涉及天冬酰胺内肽酶介导的从较大前体蛋白的加工。
