Leuze Michael R, Karpinets Tatiana V, Syed Mustafa H, Beliaev Alexander S, Uberbacher Edward C
Computer Science and Mathematics Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.
Gene Regul Syst Bio. 2012;6:93-107. doi: 10.4137/GRSB.S9357. Epub 2012 May 30.
Bacterial gene regulation involves transcription factors (TF) that bind to DNA recognition sequences in operon promoters. These recognition sequences, many of which are palindromic, are known as regulatory elements or transcription factor binding sites (TFBS). Some TFs are global regulators that can modulate the expression of hundreds of genes. In this study we examine global regulator half-sites, where a half-site, which we shall call a binding motif (BM), is one half of a palindromic TFBS. We explore the hypothesis that the number of BMs plays an important role in transcriptional regulation, examining empirical data from transcriptional profiling of the CRP and ArcA regulons. We compare the power of BM counts and of full TFBS characteristics to predict induced transcriptional activity. We find that CRP BM counts have a nonlinear effect on CRP-dependent transcriptional activity and predict this activity better than full TFBS quality or location.
细菌基因调控涉及转录因子(TF),这些转录因子会与操纵子启动子中的DNA识别序列结合。这些识别序列很多都是回文序列,被称为调控元件或转录因子结合位点(TFBS)。一些转录因子是全局调节因子,可调节数百个基因的表达。在本研究中,我们研究全局调节因子半位点,其中一个半位点,我们称之为结合基序(BM),是回文TFBS的一半。我们探讨了结合基序数量在转录调控中起重要作用这一假设,研究了来自CRP和ArcA调控子转录谱分析的实证数据。我们比较了结合基序计数和完整TFBS特征预测诱导转录活性的能力。我们发现CRP结合基序计数对CRP依赖的转录活性具有非线性影响,并且比完整TFBS的质量或位置能更好地预测这种活性。