利用配体-蛋白缀合物来监测配体-受体相互作用。
Exploiting ligand-protein conjugates to monitor ligand-receptor interactions.
机构信息
École Polytechnique Fédérale de Lausanne-EPFL, Institute of Chemical Sciences and Engineering, Institute of Bioengineering, National Centre of Competence in Research-NCCR in Chemical Biology, Lausanne, Switzerland.
出版信息
PLoS One. 2012;7(5):e37598. doi: 10.1371/journal.pone.0037598. Epub 2012 May 31.
Abstract
We introduce three assays for analyzing ligand-receptor interactions based on the specific conjugation of ligands to SNAP-tag fusion proteins. Conjugation of ligands to different SNAP-tag fusions permits the validation of suspected interactions in cell extracts and fixed cells as well as the establishment of high-throughput assays. The different assays allow the analysis of strong and weak interactions. Conversion of ligands into SNAP-tag substrates thus provides access to a powerful toolbox for the analysis of their interactions with proteins.
摘要
我们介绍了三种基于配体特异性连接到 SNAP 标签融合蛋白的配体-受体相互作用分析的测定方法。不同的 SNAP 标签融合物与配体的连接，允许在细胞提取物和固定细胞中验证可疑的相互作用，以及建立高通量测定方法。不同的测定方法可以分析强相互作用和弱相互作用。将配体转化为 SNAP 标签底物，因此为分析它们与蛋白质的相互作用提供了一个强大的工具箱。
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