Department of Surgery, the Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Xenotransplantation. 2012 May-Jun;19(3):159-65. doi: 10.1111/j.1399-3089.2012.00702.x.
To investigate the applicability of inbred Wuzhishan (WZS) miniature pigs for porcine islet isolation and purification.
Islet isolation and purification was conducted on adult (1-yr-old), male inbred WZS miniature pigs and age- and sex-matched market pigs obtained from a local slaughterhouse (control group). Pancreata were excised, and islet isolation was carried out by static digestion and discontinuous gradient centrifugation. Viability of the purified islets was tested by radioimmunochemistry assay to measure glucose-induced insulin release in culture and transplantation in an in vivo study.
The anatomical structure of the WZS miniature pig pancreas was more similar to the human pancreas than that of the market pig. Islet yield of the WZS miniature pigs' pancreata was significantly higher than that of the market pigs (6078 ± 1105 vs. 2500 ± 625 islet equivalents [IEQ]/g). In vitro study demonstrated that the islets isolated from WZS miniature pigs were viable, as they efficiently responded to glucose challenge. In vivo study showed that the islets from both groups could cure the diabetic rat with the survival varied from 3 to 5 days (median, 4.3 days) and 2-4 days (median, 3.6 days) in experimental group and control group, respectively.
Wuzhishan miniature pig pancreas may be a feasible source of islets for xenotransplantation.
研究近交五指山（WZS）小型猪是否适用于猪胰岛的分离和纯化。
对成年（1 岁）雄性近交 WZS 小型猪和从当地屠宰场获得的同龄和同性别商品猪（对照组）的胰岛进行分离和纯化。切除胰腺，通过静态消化和不连续梯度离心进行胰岛分离。通过放射免疫化学测定法检测纯化胰岛的活力，以测量培养和体内移植过程中葡萄糖诱导的胰岛素释放。
WZS 小型猪胰腺的解剖结构与人类胰腺更相似，而商品猪的胰腺则不相似。WZS 小型猪胰腺的胰岛产量明显高于商品猪（6078±1105 个胰岛当量 [IEQ]/g 比 2500±625 个胰岛当量/克）。体外研究表明，从 WZS 小型猪分离的胰岛具有活力，因为它们对葡萄糖刺激有有效反应。体内研究表明，两组胰岛均可治愈糖尿病大鼠，实验组的生存时间从 3 到 5 天不等（中位数为 4.3 天），对照组的生存时间为 2 到 4 天（中位数为 3.6 天）。
五指山小型猪胰腺可能是异种移植胰岛的可行来源。
