Department of Animal and Poultry Science, College of Agriculture and Bioresources, University of Saskatchewan , Saskatoon, SK, Canada.
J Agric Food Chem. 2012 Jul 11;60(27):6846-54. doi: 10.1021/jf3009487. Epub 2012 Jun 26.
With an available processing technology (fractionation), coproducts from bioethanol processing (wheat dried distillers grains with solubles, DDGS) could be fractionated to a desired/optimal chemical and nutrient profile. There is no study, to the author's knowledge, on manipulating nutrient profiles through fractionation processing in bioethanol coproducts in ruminants. The objectives of this study were to investigate the effect of fractionation processing of a coproduct from bioethanol processing (wheat DDGS) on the metabolic characteristics of the proteins and to study the effects of fractionation processing on the magnitude of changes in chemical and nutrient supply to ruminants by comparing chemical and nutrient characterization, in situ rumen degradation kinetics, truly absorbed protein supply, and protein degraded balance among different fractions of coproduct of wheat DDGS. In this study, wheat DDGS was dry fractionationed into A, B, C, and D fractions according to particle size, gravity, and protein and fiber contents. The results showed that the fractionation processing changed wheat DDGS chemical and nutrient profiles. NDF and ADF increased from fraction A to D (NDF, from 330 to 424; ADF, from 135 to 175 g/kg DM). Subsequently, CP decreased (CP, from 499 to 363 g/kg DM), whereas soluble CP, NPN, and carbohydrate increased (SCP, from 247 to 304 g/kg CP; NPN, from 476 to 943 g/kg SCP; CHO, from 409 to 538 g/kg DM) from fraction A to D. The CNCPS protein and carbohydrate subfractions were also changed by the fractionation processing. Effective degradability of DM and CP and total digestible protein decreased from fraction A to D (EDDM, from 734 to 649; EDCP, from 321 to 241; TDP, from 442 to 312 g/kg DM). Total truly absorbed protein in the small intestine decreased from fraction A to D (DVE value, from 186 to 124 g/kg DM; MP in NRC-2001, from 193 to 136 g/kg DM). Degraded protein balance decreased from wheat DDGS fractions A-D (DPB in the DVE/OEB system, from 245 to 161 g/kg DM; DPB in NRC-2001, from 242 to 158 g/kg DM). The fractionation processing had a great impact on the chemical and nutrition profiles. Total truly digested and absorbed protein supply and degraded protein balance were decreased. The processing relatively optimized the protein degraded balance of the coproducts to dairy cattle. Compared with the original wheat DDGS (without fractionation), fractionation processing decreased truly absorbed protein supply of DVE and MP values. In conclusion, fractionation processing can be used to manipulate the nutrient supply and N-to-energy degradation synchronization ratio of coproducts from bioethanol processing. Among the fractions, fraction A was the best in terms of its highest truly absorbed protein DVE and MP values. Fractionation processing has great potential to fractionate a coproduct into a desired and optimal chemical and nutrient profile. To the author's knowledge, this is the first paper to show that with fractionation processing, the coproducts from bioethanol processing (wheat DDGS) could be manipulated to provide a desired/optimized nutrient supply to ruminants.
利用现有的加工技术(分馏),可以将生物乙醇加工的副产物(小麦干酒糟及其可溶物,DDGS)分馏成所需/最佳的化学和营养成分。据作者所知,目前还没有关于通过分馏加工来控制反刍动物生物乙醇副产物中营养成分的研究。本研究的目的是研究分馏加工生物乙醇加工副产物(小麦 DDGS)对蛋白质代谢特性的影响,并通过比较不同 DDGS 副产物的化学和营养特性、原位瘤胃降解动力学、真正可吸收的蛋白质供应以及蛋白质降解平衡来研究分馏加工对反刍动物化学和营养供应变化幅度的影响。在这项研究中,根据颗粒大小、重力和蛋白质和纤维含量,将小麦 DDGS 干法分馏成 A、B、C 和 D 四个部分。结果表明,分馏加工改变了小麦 DDGS 的化学和营养成分。NDF 和 ADF 从 A 部分到 D 部分增加(NDF,从 330 增加到 424;ADF,从 135 增加到 175 g/kg DM)。随后,CP 减少(CP,从 499 减少到 363 g/kg DM),而可溶性 CP、NPN 和碳水化合物增加(SCP,从 247 增加到 304 g/kg CP;NPN,从 476 增加到 943 g/kg SCP;CHO,从 409 增加到 538 g/kg DM)从 A 部分到 D 部分。CNCPS 蛋白质和碳水化合物亚组分也受到分馏加工的影响。DM 和 CP 的有效降解率以及总可消化蛋白从 A 部分到 D 部分降低(EDDM,从 734 降低到 649;EDCP,从 321 降低到 241;TDP,从 442 降低到 312 g/kg DM)。从小肠中真正可吸收的总蛋白质减少(DVE 值,从 186 减少到 124 g/kg DM;NRC-2001 中的 MP,从 193 减少到 136 g/kg DM)。从 A 到 D 部分的 DDGS 副产物的降解蛋白平衡减少(DVE/OEB 系统中的 DPB,从 245 减少到 161 g/kg DM;NRC-2001 中的 DPB,从 242 减少到 158 g/kg DM)。分馏加工对化学和营养成分有很大影响。总真正消化和吸收的蛋白质供应以及降解蛋白质平衡减少。该加工过程相对优化了奶牛对副产品的蛋白质降解平衡。与原始的小麦 DDGS(未经分馏)相比,分馏加工降低了 DVE 和 MP 值的真正可吸收蛋白质供应。总之,分馏加工可用于操纵生物乙醇加工副产物的营养供应和 N 到能量的降解同步比。在这些部分中,A 部分在真正吸收的蛋白质 DVE 和 MP 值方面是最好的。分馏加工具有将副产物分馏成所需和最佳化学和营养成分的巨大潜力。据作者所知,这是第一篇表明通过分馏加工,生物乙醇加工的副产物(小麦 DDGS)可以被操纵以向反刍动物提供所需/优化的营养供应的论文。