Instituto de Investigaciones Bioquímicas de La Plata-INIBIOLP, CONICET-CCT La Plata-Universidad Nacional de La Plata-UNLP, La Plata, Argentina.
Mar Pollut Bull. 2012 Aug;64(8):1631-6. doi: 10.1016/j.marpolbul.2012.05.027. Epub 2012 Jun 14.
During reproduction vitellogenin (VTG) is transported to vitellogenic oocytes as a precursor of egg yolk lipovitellin (LV). As VTG synthesis is affected by environmental stressors, it is widely used as biomarker in endocrine disruption studies. However, it has seldom been employed to evaluate invertebrate hydrocarbon pollution. An ELISA with anti-LV antibody was developed to evaluate the impact of water-soluble fraction of crude oil (WSF) on Macrobrachium borellii vitellogenesis. Prawn VTG concentration was within the range reported for other crustaceans; LV values were positively correlated with gonadosomatic index (GSI). Females at different vitellogenic stages were exposed to a sub-lethal concentration of WSF for 7 days. Exposed animals with GSI>7 increased their VTG and LV titer as compared to control organisms (190% and 140%, respectively). VTG levels in M. borellii were upregulated and highly sensitive to WSF exposure. This assay could be employed as a biomarker for freshwater hydrocarbon pollution.
在生殖过程中，卵黄蛋白原（VTG）作为卵黄脂卵黄蛋白（LV）的前体被运输到卵黄生成的卵母细胞中。由于 VTG 的合成受到环境胁迫因素的影响，因此它被广泛用作内分泌干扰研究中的生物标志物。然而，它很少被用于评估无脊椎动物的碳氢化合物污染。本研究利用抗 LV 抗体建立了 ELISA 方法，以评估原油水溶性馏分（WSF）对罗氏沼虾卵黄发生的影响。对虾 VTG 浓度处于其他甲壳类动物报道的范围内；LV 值与性腺指数（GSI）呈正相关。处于不同卵黄发生阶段的雌性罗氏沼虾在亚致死浓度的 WSF 中暴露 7 天。与对照组相比，GSI>7 的暴露动物的 VTG 和 LV 滴度分别增加了 190%和 140%。罗氏沼虾的 VTG 水平上调，对 WSF 暴露高度敏感。该检测方法可作为淡水碳氢化合物污染的生物标志物。
