School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637459, Singapore.
J Chromatogr A. 2012 Aug 10;1250:227-49. doi: 10.1016/j.chroma.2012.05.037. Epub 2012 May 16.
Preparative supercritical fluid chromatography (prep-SFC) is an important separation process in the chromatographers toolbox. Owing to the unique properties of the mobile phase, which is predominantly CO(2), the behavior of SFC is markedly different from high performance liquid chromatography (HPLC). This review article focuses on the scale-up of preparative chromatography. The basics of SFC, with particular focus on highlighting the key differences between SFC and HPLC, are introduced. Then, a framework for rational design of prep-SFC is proposed. This framework is based on obtaining basic system parameters from analytical scale equipment, i.e., with very small amount of material, and performing design and optimization in silico to evaluate process performance and to identify operating conditions for scale-up. The tools required to obtain the input parameters such as adsorption isotherms are discussed and the development of the design and optimization framework is elaborated. Examples from the literature which use this approach for successful scale-up are provided. Finally the design of multi-column SFC systems is discussed.
制备型超临界流体色谱（prep-SFC）是色谱学家工具包中的重要分离过程。由于流动相（主要是 CO(2)）的独特性质，SFC 的行为与高效液相色谱（HPLC）明显不同。本文重点介绍制备型色谱的放大。介绍了 SFC 的基础知识，特别强调了 SFC 和 HPLC 之间的关键区别。然后，提出了制备型 SFC 的合理设计框架。该框架基于从分析规模设备（即使用非常少量的材料）获得基本系统参数，并在计算机中进行设计和优化，以评估工艺性能并确定放大的操作条件。讨论了获得吸附等温线等输入参数所需的工具，并详细阐述了设计和优化框架的开发。提供了使用这种方法成功放大的文献示例。最后讨论了多柱 SFC 系统的设计。
