[磷酸化对大脑中丙酮酸脱氢酶复合物介导的丙酮酸氧化及不依赖辅酶A和烟酰胺腺嘌呤二核苷酸的周转的影响]
[The effect of phosphorylation on oxidative and CoA- and NAD+-independent turnover of pyruvate by pyruvate dehydrogenase complex in the brain].
作者信息
Nemeria N S, Ban'kovskiĭ A A, Kravchuk R I, Ostrovskiĭ Iu M
出版信息
Ukr Biokhim Zh (1978). 1990 Sep-Oct;62(5):55-61.
It was shown that in the presence of ATP and Mg2+ the phosphorylation of the partially purified pyruvate dehydrogenase complex and the enzyme in isolated brain mitochondria inhibited the oxidative activity of the pyruvate dehydrogenase complex. The phosphorylation did no affect essentially the nonoxidative decarboxylation of pyruvate to form CO2 and acetaldehyde. In native mitochondria from the bovine brain the nonoxidative activity of the pyruvate dehydrogenase complex reached about 10% as compared to the oxidative activity of enzyme.
结果表明，在ATP和Mg2+存在的情况下，部分纯化的丙酮酸脱氢酶复合物以及分离的脑线粒体中的该酶发生磷酸化，会抑制丙酮酸脱氢酶复合物的氧化活性。磷酸化对丙酮酸非氧化脱羧生成CO2和乙醛的过程基本没有影响。在牛脑的天然线粒体中，丙酮酸脱氢酶复合物的非氧化活性约为该酶氧化活性的10%。
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