Laboratory of Pathology, Beijing Institute of Radiation Medicine, Beijing, China.
Anal Bioanal Chem. 2012 Jul;404(1):69-78. doi: 10.1007/s00216-012-6115-3. Epub 2012 Jun 17.
There has been growing public concern regarding exposure to microwave fields as a potential human health hazard. This study aimed to identify sensitive biochemical indexes for the detection of injury induced by microwave exposure. Male Wistar rats were exposed to microwaves for 6 min per day, 5 days per week over a period of 1 month at an average power density of 5 mW/cm(2) (specific absorption rate of 2.1 W/kg). Urine specimens were collected over 24 h in metabolic cages at 7 days, 21 days, 2 months, and 6 months after exposure. (1)H NMR spectroscopy data were analyzed using multivariate statistical techniques. Urine metabolic profiles of rats after long-term microwave exposure were significantly differentiated from those of sham-treated controls using principal component analysis or partial least squares discriminant analysis. Significant differences in low molecular weight metabolites (acetate, succinate, citrate, ketoglutarate, glucose, taurine, phenylalanine, tyrosine, and hippurate) were identified in the 5 mW/cm(2) microwave exposure group compared with the sham-treated controls at 7 days, 21 days, and 2 months. Metabolites returned to normal levels by 6 months after exposure. These data indicated that these metabolites were related to the perturbations of energy metabolism particularly in the tricarboxylic acid cycle, and the metabolism of amino acids, monoamines, and choline in urine represent potential indexes for the detection of injury induced by long-term microwave exposure.
公众越来越关注微波场暴露作为一种潜在的人类健康危害。本研究旨在确定敏感的生化指标，以检测微波暴露引起的损伤。雄性 Wistar 大鼠每天暴露于微波中 6 分钟，每周 5 天，持续 1 个月，平均功率密度为 5 mW/cm²（比吸收率为 2.1 W/kg）。暴露后 7 天、21 天、2 个月和 6 个月，在代谢笼中收集 24 小时尿液标本。使用多元统计技术分析 (1)H NMR 光谱数据。使用主成分分析或偏最小二乘判别分析，将长期微波暴露后大鼠的尿液代谢谱与假处理对照组进行显著区分。与假处理对照组相比，在 5 mW/cm²微波暴露组中，在 7 天、21 天和 2 个月时，低分子量代谢物（乙酸盐、琥珀酸盐、柠檬酸盐、酮戊二酸、葡萄糖、牛磺酸、苯丙氨酸、酪氨酸和马尿酸）有显著差异。暴露 6 个月后，代谢物恢复正常水平。这些数据表明，这些代谢物与能量代谢的紊乱有关，特别是三羧酸循环中的代谢紊乱，以及尿液中氨基酸、单胺和胆碱的代谢，代表了检测长期微波暴露引起的损伤的潜在指标。
