Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Sankt-Petersburg, Russia.
Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Sankt-Petersburg, Russia.
Environ Toxicol Pharmacol. 2012 Sep;34(2):334-337. doi: 10.1016/j.etap.2012.05.006. Epub 2012 May 29.
The present study was designed to examine an ability of inorganic fluoride (F) to induce oxidative stress and energy depletion in the rat erythrocytes in vitro. Accumulation of ROS and alterations in glutathione (GSH) and ATP contents were estimated in the cells incubated with 0.1-10mM NaF for 1, 5 and 24h. Exposure of the rat erythrocytes to NaF was accompanied by progressive accumulation of peroxides, while superoxide (O(2)(-)) production was insignificant. Intracellular GSH content was reduced following 5-h incubation, but considerably elevated after 24h, although GSH/GSSG ratio decreased in both cases. ATP concentration in the NaF-treated cell exhibited a dose- and time-dependent decline, diminishing to extremely low levels within 24h. Thus, exposure of the rat erythrocytes to NaF leads to impairment of the cellular antioxidant system and severe energy depletion, the latter probably being the primary toxic effect.
本研究旨在考察无机氟化物 (F) 在体外诱导大鼠红细胞氧化应激和能量耗竭的能力。将细胞与 0.1-10mM NaF 孵育 1、5 和 24h 后，评估 ROS 的积累以及谷胱甘肽 (GSH) 和 ATP 含量的变化。NaF 暴露于大鼠红细胞中伴随着过氧化物的逐渐积累，而超氧化物 (O(2)(-)) 的产生则微不足道。孵育 5 小时后，细胞内 GSH 含量减少，但 24 小时后明显升高，尽管两种情况下 GSH/GSSG 比值均降低。NaF 处理细胞中的 ATP 浓度呈剂量和时间依赖性下降，24 小时内降至极低水平。因此，NaF 暴露于大鼠红细胞中会导致细胞抗氧化系统受损和严重的能量耗竭，后者可能是主要的毒性作用。
