Department of Chemical Engineering, Sungkyunkwan University, 300 Cheoncheondong, Jangangu, Suwon, Gyeonggido 440-746, South Korea.
Environ Toxicol Pharmacol. 2012 Sep;34(2):345-350. doi: 10.1016/j.etap.2012.05.011. Epub 2012 Jun 1.
The degradation of several antibiotics (tetracycline, sulfathiazole, ampicillin) was performed with immobilized bacterial cells containing the glutathione S-transferases (GSTs). Antibiotics in animal feed contaminated wastewater usually inhibit the growth of microorganisms that treat the wastewater, so a bio-friendly treatment method is required. Therefore, we have shown that the inhibitory effects of antibiotics on bacteria were reduced by microorganisms containing detoxifying enzyme GSTs by using a cell immobilizing method in a bioreactor. The initial concentrations of tetracycline, sulfathiazole and ampicillin were 100mg/L, 100mg/L and 50mg/L respectively, which are typical of the range detected in pig feed in Korea. In the results, we observed the removal efficiency of tetracycline to be almost 70% with Staphylococcus epidermidis in the bioreactor, suggesting that this method of antibiotic removal is worthy of further study.
采用含有谷胱甘肽 S-转移酶（GSTs）的固定化细菌细胞对几种抗生素（四环素、磺胺噻唑、氨苄西林）进行了降解。动物饲料废水中的抗生素通常会抑制处理废水的微生物的生长，因此需要一种生物友好的处理方法。因此，我们已经表明，通过在生物反应器中使用细胞固定化方法，含有解毒酶 GST 的微生物可以降低抗生素对细菌的抑制作用。四环素、磺胺噻唑和氨苄西林的初始浓度分别为 100mg/L、100mg/L 和 50mg/L，这是韩国猪饲料中检测到的典型浓度范围。结果表明，在生物反应器中，表皮葡萄球菌对四环素的去除效率几乎达到 70%，这表明这种抗生素去除方法值得进一步研究。
