Department of Chemical and Pharmaceutical Engineering, College of Chemical Engineering, Huaqiao University, Xiamen 361021, Fujian Province, PR China.
Prog Neuropsychopharmacol Biol Psychiatry. 2012 Oct 1;39(1):175-81. doi: 10.1016/j.pnpbp.2012.06.009. Epub 2012 Jun 15.
Our previous study demonstrated that the citrus bioflavonoid naringenin ameliorated behavioral alterations via the central serotonergic and noradrenergic systems in the tail suspension test (TST) induced mice. To better understand its pharmacological activity, mice were submitted to three 6min-TSTs one week apart (Day 1: test, Day 7: retest 1, Day 14: retest 2) followed by hippocampal glucocorticoid receptor (GR), monoamine neurotransmitters and serum corticosterone measurement. The results suggested that repeated TST detected the gradual increase in the efficacy of naringenin over time, additionally 1-day (20 mg/kg), 7-day (10, 20 mg/kg) and 14-day (5, 10, 20 mg/kg) naringenin treatment markedly decreased the immobility time. Moreover, administration of naringenin for 14 days (20 mg/kg) increased hippocampal serotonin (5-HT), norepinephrine (NE) and GR levels, and reduced serum corticosterone levels in mice exposed to the repeated TST. Overall, the present study indicated that the re-exposure would facilitate the detection of the anti-immobility effects of antidepressant drugs in the mouse TST, and clearly demonstrated that the antidepressant-like effect of naringenin may be mediated by an interaction with neuroendocrine and neurochemical systems.
我们之前的研究表明，柚皮苷通过调节中枢 5-羟色胺能和去甲肾上腺素能系统改善了悬尾试验诱导的小鼠的行为改变。为了更好地理解其药理学活性，将小鼠进行三次为期 6 分钟的悬尾试验，间隔一周（第 1 天：测试，第 7 天：第 1 次重测，第 14 天：第 2 次重测），随后测量海马糖皮质激素受体（GR）、单胺神经递质和血清皮质酮。结果表明，重复悬尾试验检测到柚皮苷的疗效随时间逐渐增加，此外，1 天（20mg/kg）、7 天（10、20mg/kg）和 14 天（5、10、20mg/kg）柚皮苷治疗显著减少了不动时间。此外，14 天（20mg/kg）的柚皮苷给药增加了反复悬尾试验暴露小鼠海马中的 5-羟色胺（5-HT）、去甲肾上腺素（NE）和 GR 水平，并降低了血清皮质酮水平。总之，本研究表明，重新暴露会促进在小鼠悬尾试验中检测抗不动作用的抗抑郁药物，并且明确表明，柚皮苷的抗抑郁样作用可能是通过与神经内分泌和神经化学系统的相互作用介导的。
