L'Oréal Research and Innovation, Clichy, France.
Indian J Dermatol Venereol Leprol. 2012 Jun;78 Suppl 1:S15-23. doi: 10.4103/0378-6323.97351.
Cutaneous damages such as sunburn, pigmentation, and photoaging are known to be induced by acute as well as repetitive sun exposure. Not only for basic research, but also for the design of the most efficient photoprotection, it is crucial to understand and identify the early biological events occurring after ultraviolet (UV) exposure. Reconstructed human skin models provide excellent and reliable in vitro tools to study the UV-induced alterations of the different skin cell types, keratinocytes, fibroblasts, and melanocytes in a dose- and time-dependent manner. Using different in vitro human skin models, the effects of UV light (UVB and UVA) were investigated. UVB-induced damages are essentially epidermal, with the typical sunburn cells and DNA lesions, whereas UVA radiation-induced damages are mostly located within the dermal compartment. Pigmentation can also be obtained after solar simulated radiation exposure of pigmented reconstructed skin model. Those models are also highly adequate to assess the potential of sunscreens to protect the skin from UV-associated damage, sunburn reaction, photoaging, and pigmentation. The results showed that an effective photoprotection is provided by broad-spectrum sunscreens with a potent absorption in both UVB and UVA ranges.
已知皮肤损伤，如晒伤、色素沉着和光老化，是由急性和反复的阳光照射引起的。为了基础研究，也为了设计最有效的光保护，了解和识别紫外线（UV）暴露后发生的早期生物学事件至关重要。重建的人体皮肤模型为研究不同皮肤细胞类型（角质形成细胞、成纤维细胞和黑素细胞）在剂量和时间依赖性方面的 UV 诱导改变提供了极好的、可靠的体外工具。使用不同的体外人体皮肤模型，研究了 UV 光（UVB 和 UVA）的作用。UVB 诱导的损伤主要是表皮的，具有典型的晒伤细胞和 DNA 损伤，而 UVA 辐射诱导的损伤主要位于真皮隔室。色素沉着也可以在色素沉着重建皮肤模型接受太阳模拟辐射后获得。这些模型也非常适合评估防晒霜保护皮肤免受与紫外线相关的损伤、晒伤反应、光老化和色素沉着的潜力。结果表明，广谱防晒霜在 UVB 和 UVA 范围内具有强大的吸收能力，可提供有效的光保护。
