Department of Urology, Guangdong Key Laboratory of Clinical Molecular Medicine and Diagnostics, Guangzhou First Municipal People's Hospital, Affiliated Guangzhou Medical College, Guangzhou 510180, China.
FEBS Lett. 2012 Jul 30;586(16):2451-8. doi: 10.1016/j.febslet.2012.06.003. Epub 2012 Jun 16.
To investigate the mechanism by which peroxiredoxin III (PRDX3) is altered in human prostate cancer (PCa), we used microRNA (miRNA) target prediction program and miRNA microarray to predict and identify miR-23b as a candidate miRNA that targets PRDX3. We showed that miR-23b suppresses PRDX3 protein expression in human DU145 cells under normal and hypoxic conditions. Additionally, the clinical significance of miR-23b and PRDX3 expression in PCa patients was also confirmed. In conclusion, our data suggest that the effects of PRDX3 in PCa progression may be caused by the regulation function of miR-23b, and consequently, miR-23b may be involved in the response of PCa cells to hypoxia stress.
为了研究过氧化物酶 III (PRDX3) 在人前列腺癌 (PCa) 中改变的机制，我们使用 microRNA (miRNA) 靶标预测程序和 miRNA 微阵列来预测和鉴定 miR-23b 作为靶向 PRDX3 的候选 miRNA。我们表明，miR-23b 在正常和缺氧条件下抑制人 DU145 细胞中的 PRDX3 蛋白表达。此外，还证实了 miR-23b 和 PRDX3 在 PCa 患者中的表达的临床意义。总之，我们的数据表明，PRDX3 在 PCa 进展中的作用可能是由 miR-23b 的调节功能引起的，因此，miR-23b 可能参与了 PCa 细胞对缺氧应激的反应。
