胃腺癌中表皮生长因子受体的磷酸化和 7 号染色体三倍体。

Phosphorylation of epidermal growth factor receptor and chromosome 7 polysomy in gastric adenocarcinoma.

机构信息

Department of Pathology, Shanghai Jiao-Tong University School of Medicine, Shanghai, China.

出版信息

J Dig Dis. 2012 Jul;13(7):350-9. doi: 10.1111/j.1751-2980.2012.00597.x.

DOI:10.1111/j.1751-2980.2012.00597.x

PMID:22713084

Abstract

OBJECTIVE

To investigate the phosphorylation of epidermal growth factor receptor (EGFR) and its potentially associated chromosomal aberrations in gastric adenocarcinoma.

METHODS

Phosphorylated EGFR (pEGFR) was detected by immunohistochemistry on 145 specimens including 60 tumoral, 60 non-tumoral, 12 tumor-adjacent intramucosal dysplasia from patients with gastric adenocarcinoma and 13 mucosae from cancer-free patients. EGFR gene amplification and chromosome 7 (Chr-7) polysomy were detected by fluorescence in situ hybridization.

RESULTS

Positivity of pEGFR was found in 50 tumoral (83.3%) and 42 non-tumoral specimens (70.0%). There was an association between tumoral and non-tumoral zones on immunostains of pEGFR (r = 0.353, P = 0.006). Nuclear pEGFR usually presented in mucosae with Helicobacter pylori infection, stromal reaction or vascular invasion. Cytoplasmic pEGFR was correlated with local cancer extension (r = 0.337, P = 0.014) and inversely related with gastrokine 2, which had been previously detected in the same specimens. Eleven intramucosal dysplastic specimens were also positive for pEGFR while 13 mucosae from cancer-free patients were all negative. No EGFR gene amplification was observed. However, seven tumor specimens showed Chr-7 polysomy (11.7%) in which 5 were strongly positive for pEGFR.

CONCLUSIONS

EGFR phosphorylation may be one of the mechanisms that promote tumor initiation and expansion in gastric adenocarcinoma. Detection of pEGFR with analysis of its nuclear or cytoplasmic patterns could be clinicopathologically valuable. Chr-7 polysomy may partially contribute to EGFR activation in gastric adenocarcinoma, although its role does not predominate.

摘要

目的

研究胃腺癌中表皮生长因子受体（EGFR）的磷酸化及其潜在的染色体畸变。

方法

采用免疫组化法检测 145 例标本中的磷酸化 EGFR（pEGFR），包括 60 例肿瘤组织、60 例非肿瘤组织、12 例肿瘤旁黏膜上皮内异型增生组织和 13 例无癌患者的黏膜组织。采用荧光原位杂交法检测 EGFR 基因扩增和 7 号染色体（Chr-7）多倍体。

结果

pEGFR 在 50 例肿瘤组织（83.3%）和 42 例非肿瘤组织（70.0%）中呈阳性。肿瘤和非肿瘤组织 pEGFR 免疫染色之间存在相关性（r = 0.353，P = 0.006）。核 pEGFR 通常存在于幽门螺杆菌感染、间质反应或血管侵犯的黏膜中。细胞质 pEGFR 与局部癌症扩展相关（r = 0.337，P = 0.014），与胃泌素 2 呈负相关，胃泌素 2 曾在同一标本中被检测到。11 例黏膜上皮内异型增生组织也呈 pEGFR 阳性，而 13 例无癌患者的黏膜组织均为阴性。未观察到 EGFR 基因扩增。然而，7 例肿瘤标本存在 Chr-7 多倍体（11.7%），其中 5 例 pEGFR 强阳性。