Seo Seung Ree, Kim Jin-Chul
College of Biomedical Science and Institute of Bioscience and Biotechnology, Kangwon National University , Hyoja 2 dong, Chunchon, Kangwon-do , Korea.
Drug Dev Ind Pharm. 2013 Dec;39(12):1921-7. doi: 10.3109/03639045.2012.696655. Epub 2012 Jun 19.
Interpenetrating network (IPN) beads composed of alginate and poly(vinyl alcohol)-epoxypropoxy coumarin (PVA-EPC) conjugate were prepared by dropping the mixture solution into CaCl2 solution under UV irradiation (λ = 365 nm). EPC residues of PVA-EPC could readily be dimerized by λ = 365 nm and de-dimerized by λ= 254 nm even in the presence of alginate. In addition, the viscosity of the mixture solution increased with the time of UV irradiation due to the photo-dimerization. The percentage release in 360 min of FITC-dextran loaded in the beads, which were prepared using the mixture solutions of which alginate/PVA-EPC concentrations were 0.5%/0.5%, 0.5%/1%, and 0.5%/2.5%, were about 2%, 11%, and 5%, respectively. Interestingly, in case of beads prepared using the mixture solution of which alginate/PVA-EPC concentration was 0.5%/2.5%, the release was a little but significantly promoted by the irradiation of λ= 254 nm.
通过在紫外光（λ = 365 nm）照射下将混合溶液滴入氯化钙溶液中，制备了由海藻酸钠和聚乙烯醇 - 环氧丙氧基香豆素（PVA - EPC）共轭物组成的互穿网络（IPN）珠。即使存在海藻酸钠，PVA - EPC中的EPC残基也能在λ = 365 nm下容易地二聚化，并在λ = 254 nm下解二聚化。此外，由于光二聚化，混合溶液的粘度随紫外光照射时间增加。使用海藻酸钠/PVA - EPC浓度分别为0.5%/0.5%、0.5%/1%和0.5%/2.5%的混合溶液制备的载有FITC - 葡聚糖的珠在360分钟内的释放百分比分别约为2%、11%和5%。有趣的是，对于使用海藻酸钠/PVA - EPC浓度为0.5%/2.5%的混合溶液制备的珠，λ = 254 nm的照射对释放有轻微但显著的促进作用。
