γδ T 细胞在胃癌患者的外周血中增加。
γδ T cells are increased in the peripheral blood of patients with gastric cancer.
机构信息
Department of Laboratory Medicine, University of Daegu, School of Medicine, Daegu, Korea.
出版信息
Clin Chim Acta. 2012 Oct 9;413(19-20):1495-9. doi: 10.1016/j.cca.2012.06.016. Epub 2012 Jun 17.
BACKGROUND
γδ T cells are implicated in immunoregulation. However, little is known about the characteristics of γδ T cells in gastric cancer. In this study, we assessed the incidence of γδ T cells and lymphocyte subsets in the peripheral blood of gastric cancer patients.
METHODS
We enrolled 48 patients and 49 healthy controls. The γδ T cells, lymphocyte subsets were analyzed with flow cytometry.
RESULTS
The mean percentage of γδ T cells in patients with gastric cancer was 5.0±3.4% and for controls 2.3±1.6%. Twenty (41.7%) of the 48 patients with gastric cancer had a high percentage (more than 5%) of peripheral blood γδ T cells, while 4 (8.2%) of the 49 controls did. The percentage of CD3⁺ T cells were elevated in gastric cancer compared to controls (P=0.007). The CD4/CD8 ratio increased in gastric cancer (P=0.311). The percentage of CD3⁺CD4⁻CD8⁻ T cells increased in gastric cancer compared to controls (P=0.004).
CONCLUSION
The proportion of γδ T cells in the peripheral blood of gastric cancer patients was significantly higher in comparison to that in the healthy controls. Our findings suggest that increased proportion of peripheral γδ T cells may explain anti-tumor immunity against gastric cancer partly.
背景
γδ T 细胞在免疫调节中起作用。然而，人们对胃癌中 γδ T 细胞的特征知之甚少。在这项研究中，我们评估了胃癌患者外周血中 γδ T 细胞和淋巴细胞亚群的发生率。
方法
我们纳入了 48 名胃癌患者和 49 名健康对照者。采用流式细胞术分析 γδ T 细胞和淋巴细胞亚群。
结果
胃癌患者外周血 γδ T 细胞的平均百分比为 5.0±3.4%，健康对照者为 2.3±1.6%。48 名胃癌患者中有 20 名（41.7%）外周血 γδ T 细胞百分比较高（大于 5%），而 49 名健康对照者中有 4 名（8.2%）。与健康对照组相比，胃癌患者的 CD3⁺ T 细胞百分比升高（P=0.007）。胃癌患者的 CD4/CD8 比值升高（P=0.311）。与健康对照组相比，胃癌患者的 CD3⁺CD4⁻CD8⁻ T 细胞百分比升高（P=0.004）。
结论
与健康对照组相比，胃癌患者外周血中 γδ T 细胞的比例明显更高。我们的研究结果表明，外周 γδ T 细胞比例的增加可能部分解释了针对胃癌的抗肿瘤免疫。
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