Medical Proteomics Unit, Office for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
Eur J Pharmacol. 2012 Aug 15;689(1-3):219-25. doi: 10.1016/j.ejphar.2012.06.012. Epub 2012 Jun 17.
Dissolution therapy of calcium oxalate monohydrate (COM) kidney stone disease has not yet been implemented due to a lack of well characterized COM dissolution agents. The present study therefore aimed to identify potential COM crystal dissolution compounds. COM crystals were treated with deionized water (negative control), 5 mM EDTA (positive control), 5 mM sodium citrate, or 5mM sodium phosphate. COM crystal dissolution activities of these compounds were evaluated by phase-contrast and video-assisted microscopic examinations, semi-quantitative analysis of crystal size, number and total mass, and spectrophotometric oxalate-dissolution assay. In addition, effects of these compounds on detachment of COM crystals, which adhered tightly onto renal tubular cell surface, were also investigated. The results showed that citrate, not phosphate, had a significant dissolution effect on COM crystals as demonstrated by significant reduction of crystal size (approximately 37% decrease), crystal number (approximately 53% decrease) and total crystal mass (approximately 72% decrease) compared to blank and negative controls. Spectrophotometric oxalate-dissolution assay successfully confirmed the COM crystal dissolution property of citrate. Moreover, citrate could detach up to 85% of the adherent COM crystals from renal tubular cell surface. These data indicate that citrate is better than phosphate for dissolution and detachment of COM crystals.
由于缺乏特征明确的 COM 溶解剂，草酸钙一水合物 (COM) 肾结石疾病的溶解疗法尚未实施。因此，本研究旨在确定潜在的 COM 晶体溶解化合物。用去离子水（阴性对照）、5mM EDTA（阳性对照）、5mM 柠檬酸钠或 5mM 磷酸钠处理 COM 晶体。通过相差和视频辅助显微镜检查、晶体大小、数量和总质量的半定量分析以及分光光度草酸溶解测定评估这些化合物对 COM 晶体的溶解活性。此外，还研究了这些化合物对紧密附着在肾小管细胞表面的 COM 晶体的脱落作用。结果表明，与空白和阴性对照相比，柠檬酸钠而非磷酸钠对 COM 晶体具有显著的溶解作用，表现为晶体大小（约 37%减少）、晶体数量（约 53%减少）和总晶体质量（约 72%减少）显著降低。分光光度草酸溶解测定成功证实了柠檬酸钠对 COM 晶体的溶解性质。此外，柠檬酸钠可从肾小管细胞表面脱落多达 85%的附着 COM 晶体。这些数据表明，柠檬酸钠在溶解和脱落 COM 晶体方面优于磷酸钠。
