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蛙眼单个视杆外段的 X 射线衍射研究。

An X-ray diffraction study on a single rod outer segment from frog retina.

机构信息

Japan Synchrotron Radiation Research Institute, SPring-8, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5198, Japan.

出版信息

J Synchrotron Radiat. 2012 Jul;19(Pt 4):574-8. doi: 10.1107/S0909049512018535. Epub 2012 May 18.

DOI:10.1107/S0909049512018535
PMID:22713892
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3380658/
Abstract

X-ray diffraction patterns were recorded from isolated single rod outer segments of frog. The outer segments in Ringer's solution were exposed to a 6 µm microbeam (15 keV) at the BL40XU beamline, SPring-8. The diffraction pattern demonstrated a remarkable regularity in the stacking and flatness of the disk membranes. The electron density profile calculated from the intensity of up to tenth-order reflections showed a pair of bilayers that comprise a disk membrane. The structure of the disk membrane and the changes in the profile on swelling generally agreed with previous reports. Radiation damage was significant with an irradiation of 5 × 10(5) Gy which is much lower than the known damaging dose on proteins at the liquid-nitrogen temperature.

摘要

我们从青蛙的分离的单个杆状部的外节段中记录了 X 射线衍射图谱。在 Ringer 溶液中的外节段在 SPring-8 的 BL40XU 光束线上暴露于 6μm 的微光束（15keV）下。衍射图谱显示出盘膜的堆叠和平整度的显著规律性。从高达第十阶反射强度计算出的电子密度分布显示出一对双层，其构成了盘膜。盘膜的结构和肿胀时轮廓的变化通常与以前的报告一致。辐照损伤非常显著，辐照剂量为 5×10(5)Gy，远低于在液氮温度下已知的蛋白质损伤剂量。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60a7/3380658/13955210e375/s-19-00574-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60a7/3380658/2bf89d2f332b/s-19-00574-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60a7/3380658/7e555ebfb8a9/s-19-00574-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60a7/3380658/7534e69ee374/s-19-00574-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60a7/3380658/3dcfb8737137/s-19-00574-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60a7/3380658/13955210e375/s-19-00574-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60a7/3380658/2bf89d2f332b/s-19-00574-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60a7/3380658/7e555ebfb8a9/s-19-00574-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60a7/3380658/7534e69ee374/s-19-00574-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60a7/3380658/3dcfb8737137/s-19-00574-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60a7/3380658/13955210e375/s-19-00574-fig5.jpg

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本文引用的文献

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Optical properties of in situ eye lenses measured with X-ray Talbot interferometry: a novel measure of growth processes.用 X 射线泰伯干涉法测量原位晶状体的光学特性：一种测量生长过程的新方法。
PLoS One. 2011;6(9):e25140. doi: 10.1371/journal.pone.0025140. Epub 2011 Sep 20.
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The partition of cholesterol between ordered and fluid bilayers of phosphatidylcholine: a synchrotron X-ray diffraction study.胆固醇在磷脂酰胆碱有序双层和流体双层之间的分配：一项同步加速器X射线衍射研究。
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Three-dimensional architecture of murine rod outer segments determined by cryoelectron tomography.
通过冷冻电子断层扫描确定的小鼠视杆细胞外段的三维结构
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