Division of Applied Life Science (BK21), Graduate School, Gyeongsang National University, Jinju 660-701, Korea.
J Microbiol Biotechnol. 2012 Aug;22(8):1092-100. doi: 10.4014/jmb.1110.10002.
Ten Bacillus strains with antimicrobial activities were isolated from Cheonggukjang produced at different parts in Korea. They all inhibited Listeria monocytogenes ATCC 19111 and nine inhibited Bacillus cereus ATCC 14579. Four isolates (W42, H27, SKE 12, and K21) showing strong inhibiting activities were identified as B. subtilis. B. subtilis W42 was the most inhibiting strain. The antimicrobial activity of culture supernatant from B. subtilis W42 was destroyed completely by proteinase K treatment, indicating that a bacteriocin was the responsible agent. The bacteriocin, Bac W42, was most stable at pH 7 and stable between pH 3-6 and 8-9. Bac W42 was stable up to 80°C. BHI (brain heart infusion) and TSB (tryptic soy broth) were the best media for the activity (320 AU/ml) followed by LB (160 AU/ml). Bac W42 was partially purified by column chromatographies. The specific activity was increased from 1,151.2 AU/ml to 9,043.5 AU/ml and the final yield was 26.3%. Bac W42 was 5.4 kDa in size as determined by SDS-PAGE. Bac W42 showed bactericidal activity against L. monocytogenes ATCC 19111.
从韩国不同地区生产的清曲酱中分离出 10 株具有抗菌活性的芽孢杆菌。它们均能抑制单核细胞增生李斯特菌 ATCC 19111，其中 9 株能抑制蜡状芽孢杆菌 ATCC 14579。4 株（W42、H27、SKE 12 和 K21）表现出较强抑制活性的分离物被鉴定为枯草芽孢杆菌。枯草芽孢杆菌 W42 是抑制活性最强的菌株。枯草芽孢杆菌 W42 培养上清液的抗菌活性被蛋白酶 K处理完全破坏，表明其抗菌活性是由细菌素引起的。这种细菌素命名为 Bac W42，最稳定的 pH 值为 7，在 pH 3-6 和 8-9 之间稳定。Bac W42 可耐受 80°C 的高温。BHI（脑心浸液）和 TSB（胰蛋白胨大豆肉汤）是活性（320 AU/ml）的最佳培养基，其次是 LB（160 AU/ml）。枯草芽孢杆菌 W42 通过柱层析进行部分纯化。比活力从 1,151.2 AU/ml 提高到 9,043.5 AU/ml，最终收率为 26.3%。SDS-PAGE 测定 Bac W42 的分子量为 5.4 kDa。Bac W42 对单核细胞增生李斯特菌 ATCC 19111 具有杀菌活性。
