Physical Chemistry of Biosystems, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany.
J Phys Chem B. 2012 Jul 19;116(28):8024-30. doi: 10.1021/jp212385p. Epub 2012 Jul 6.
We investigated a potential application of hydrophobic poly(n-butyl acrylate) networks (cPnBA) as substrates with tunable elasticity for culturing, maintenance, and regulation of human osteosarcoma cells (U2OS). Nanoindentation experiments with an atomic force microscope revealed that the mechanical properties of cPnBA films are maintained under aqueous conditions, confirming that the substrate elasticity can be controlled simply by the degree of cross-linking, independent from the culture medium. We found that the adhesion U2OS cells to cPnBA substrates could be improved by surface treatments such as oxgen plasma and serum proteins. To determine the strength of cell adhesion, the critical pressure to detach cells from cPnBA substrates was measured using a shock wave induced by an intensive picosecond laser pulse. A monotonic increase in the cell adhesion strength in accordance with the substrate elasticity demonstrated the potential of intrinsically hydrophobic cPnBA as a new class of substrate material with tunable mechanical properties that are not influenced by the culture medium.
我们研究了疏水性聚（正丁基丙烯酸酯）网络（cPnBA）作为具有可调弹性的基底在培养、维持和调控人骨肉瘤细胞（U2OS）方面的潜在应用。原子力显微镜的纳米压痕实验表明，cPnBA 薄膜的力学性能在水相条件下得以维持，这证实了基底弹性可以通过交联度来简单控制，与培养基无关。我们发现，cPnBA 基底的细胞黏附可以通过氧等离子体和血清蛋白等表面处理来改善。为了确定细胞黏附的强度，使用强皮秒激光脉冲产生的冲击波测量了使细胞从 cPnBA 基底上脱离所需的临界压力。细胞黏附强度与基底弹性呈单调增加，这表明具有内在疏水性的 cPnBA 作为一种具有可调机械性能的新型基底材料具有潜力，其机械性能不受培养基的影响。
