Rainville Paul D, Simeone Jennifer L, McCarthy Sean M, Smith Norman W, Cowan David, Plumb Robert S
King's College London, London, UK.
Bioanalysis. 2012 Jun;4(11):1287-97. doi: 10.4155/bio.12.78.
The movement towards environmentally friendly or green chemistry solutions has gained more prominence recently in the scientific community. One way in which scientists can address this issue is to limit the use of hazardous chemicals in their everyday processes. Therefore, the focus of this study was on the utilization of microbore-scale chromatography and nontraditional alcoholic mobile phases as an alternative approach to traditional bioanalytical LC-MS/MS assay parameters.
Replacement of the traditional narrowbore LC column with a microbore format reduced solvent consumption and produced a greater than threefold increase in S/N. The nontraditional alcoholic mobile phases, ethanol or isopropanol, produced either greater peak area counts, or S/N, for over half of the compounds evaluated, compared with the traditional organic mobile phases of acetonitrile and methanol. These nontraditional alcoholic mobile phases also showed improved capability in the removal of plasma phospholipid components from the chromatographic column. The ionizable background detected in each of the organic mobile phases utilized in this study produced a unique background that may or may not interfere with compounds undergoing analysis.
The combination of microbore columns and nontraditional alcoholic mobile phases has been shown to produce effective, alternative method conditions to traditional bioanalytical LC-MS/MS method parameters.
最近，向环境友好型或绿色化学解决方案的转变在科学界变得更加突出。科学家解决这一问题的一种方法是在日常过程中限制使用有害化学物质。因此，本研究的重点是利用微径规模色谱法和非传统的醇类流动相作为传统生物分析液相色谱-串联质谱（LC-MS/MS）分析参数的替代方法。
用微径柱替代传统的窄径液相色谱柱可减少溶剂消耗，并使信噪比提高三倍以上。与传统的乙腈和甲醇有机流动相相比，非传统的醇类流动相乙醇或异丙醇，对超过一半的评估化合物产生了更大的峰面积计数或信噪比。这些非传统的醇类流动相在从色谱柱中去除血浆磷脂成分方面也表现出更好的能力。本研究中使用的每种有机流动相检测到的可电离背景产生了独特的背景，可能会也可能不会干扰正在分析的化合物。
微径柱和非传统醇类流动相的结合已被证明能产生有效的替代方法条件，以替代传统生物分析LC-MS/MS方法参数。
