Rao J P, Agrawal P, Mohammad R, Rao S K, Reddy G R, Dechamma H J, S Suryanarayana V V
Acta Virol. 2012;56(2):91-9. doi: 10.4149/av_2012_02_91.
Recently, transgenic plants expressing immunogenic proteins of foot-and-mouth disease virus (FMDV) have been used as oral or parenteral vaccines against foot-and-mouth disease (FMD). They exhibit advantages like cost effectiveness, absence of processing, thermostability, and easy oral application. FMDV VP1 protein of single serotype has been mostly used as immunogen. Here we report the development of a bivalent vaccine with tandem-linked VP1 proteins of two serotypes, A and O, present in transgenic forage crop Crotalaria juncea. The expression of the bivalent protein in the transgenic plants was confirmed by Western blot analysis. Guinea pig reacted to orally or parenterally applied vaccine by humoral as well as cell-mediated immune responses including serum antibodies and stimulated lymphocytes, respectively. The vaccine protected the animals against a challenge with the virus of serotype A as well as O. This is the first report on the development of a bivalent FMD vaccine using a forage crop.
foot-and-mouth disease; sunnhemp; Agrobacterium tumefaciens; FMDV-VP1 gene; serotype O and A; in planta transformation; transgenic plants; bivalent vaccine.
最近，表达口蹄疫病毒（FMDV）免疫原性蛋白的转基因植物已被用作预防口蹄疫（FMD）的口服或肠胃外疫苗。它们具有成本效益、无需加工、热稳定性和易于口服应用等优点。单一血清型的FMDV VP1蛋白大多被用作免疫原。在此，我们报告了一种双价疫苗的研发情况，该疫苗由转基因饲料作物猪屎豆中两种血清型（A和O）的串联连接VP1蛋白组成。通过蛋白质免疫印迹分析证实了双价蛋白在转基因植物中的表达。豚鼠对口服或肠胃外施用的疫苗分别通过体液免疫和细胞介导的免疫反应产生反应，体液免疫反应包括血清抗体，细胞介导的免疫反应包括刺激淋巴细胞。该疫苗保护动物免受A和O血清型病毒的攻击。这是关于使用饲料作物研发双价FMD疫苗的首份报告。
口蹄疫；猪屎豆；根癌农杆菌；FMDV-VP1基因；O和A血清型；植物体内转化；转基因植物；双价疫苗
