Department of Obstetrics and Gynecology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, People's Republic of China.
Gynecol Oncol. 2012 Oct;127(1):223-30. doi: 10.1016/j.ygyno.2012.06.008. Epub 2012 Jun 18.
Wiskott-Aldrich syndrome protein family verprolin-homologous protein 1 (WAVE1) has been shown to promote cancer invasion and metastasis. However, no evidence has been found to identify the role of WAVE1 in epithelial ovarian cancer (EOC). This study aims to determine the effect of WAVE1 expression and investigate a possible relationship between WAVE1 and prognosis in EOC.
WAVE1 protein level was measured in 223 EOC specimens by immunohistochemical staining and 46 EOC specimens by Western blot analysis. Expression of WAVE1 in ovarian cancer cell lines was evaluated by Western blot analysis and immunofluorescence. Survival analysis was performed to assess the correlation between WAVE1 expression and survival.
Immunohistochemical staining and Western blot analysis showed that WAVE1 was overexpressed in EOC compared with samples from a non-invasive ovarian tumor and normal ovaries (P<0.05). Furthermore, expression of WAVE1 was significantly associated with advanced FIGO stage, poor grade, serum Ca-125 and residual tumor size (P<0.05). By Western blot analysis, WAVE1 expression was detected in four ovarian cancer cell lines. Immunofluorescence was performed to demonstrate WAVE1 expression in SKOV3 and 3AO cell lines. Survival analysis showed that patients with low WAVE1 staining had a significantly better survival compared to patients with high WAVE1 staining (P<0.05). In multivariate analysis, WAVE1 overexpression, advanced stage and suboptimal surgical debulking were independent prognostic factors of poor survival.
Our present study finds that WAVE1 overexpression is associated with an unfavorable prognosis. WAVE1 is an independent prognostic factor for EOC, which suggests that it is a novel and crucial predictor for EOC metastasis.
已证实威特霍克-奥尔德里奇综合征蛋白家族中的 verprolin 同源蛋白 1（WAVE1）可促进癌症侵袭和转移。然而，目前尚无证据表明 WAVE1 在卵巢上皮癌（EOC）中发挥作用。本研究旨在确定 WAVE1 表达的作用，并探讨 WAVE1 与 EOC 预后之间的可能关系。
通过免疫组织化学染色检测 223 例 EOC 标本和 Western blot 分析检测 46 例 EOC 标本，检测 WAVE1 蛋白水平。通过 Western blot 分析和免疫荧光评估 WAVE1 在卵巢癌细胞系中的表达。进行生存分析以评估 WAVE1 表达与生存之间的相关性。
免疫组织化学染色和 Western blot 分析显示，与非侵袭性卵巢肿瘤和正常卵巢组织相比，EOC 中 WAVE1 过度表达（P<0.05）。此外，WAVE1 的表达与 FIGO 晚期、低分化、血清 Ca-125 和残留肿瘤大小显著相关（P<0.05）。Western blot 分析检测到四种卵巢癌细胞系中均有 WAVE1 的表达。免疫荧光显示 SKOV3 和 3AO 细胞系中存在 WAVE1 表达。生存分析显示，低 WAVE1 染色患者的生存明显优于高 WAVE1 染色患者（P<0.05）。多因素分析显示，WAVE1 过表达、晚期和手术切除不彻底是不良生存的独立预后因素。
本研究发现 WAVE1 过表达与不良预后相关。WAVE1 是 EOC 的独立预后因素，提示其是 EOC 转移的一个新的重要预测因子。
