Quality of prokaryote genome assembly: indispensable issues of factors affecting prokaryote genome assembly quality.

The growing number of complete sequencing projects based on the next-generation sequencing (NGS) platforms necessitates quality evaluation. Therefore, the use of guaranteed measures such as N50, N80 and average size of contigs etc. to evaluate the quality of genome assemblies produced by ab initio methods remains vital. Herein, we prove that various treatment qualities and their influence on the whole genome products must be considered in genome assembly quality measurements.