State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
Aquat Toxicol. 2012 Oct 15;122-123:28-35. doi: 10.1016/j.aquatox.2012.05.008. Epub 2012 Jun 1.
Decabromodiphenyl ether (BDE 209), the primary component in a commonly used flame retardants, has recently been shown to be metabolized by organisms. In the present study, juvenile rainbow trout (Oncorhynchus mykiss) were exposed to BDE 209 at five nominal gradient concentrations from 50 to 1000 ng/g wet weight for 21 days via a single intraperitoneal injection. Then the liver, kidney and blood samples were collected to analyze for its debrominated, hydroxylated and methoxylated metabolites. The relationships between levels of BDE 209 metabolites in different tissues and thyroid hormone (TH) levels in plasma were evaluated. The results showed that BDE 209 could be metabolized into debrominated BDEs, methoxylated BDEs (MeO-BDEs) and hydroxylated BDEs (OH-BDEs). Levels of these three metabolites were tissue-dependent. The TH levels, including total thyroxine (TT(4)), free thyroxine (FT(4)), total triiodothyronine (TT(3)) and free triiodothyronine (FT(3)) in plasma, were significantly affected by BDE 209 metabolism. However, only FT(4) levels showed a negative correlation with MeO-BDE and OH-BDE metabolites, among which the correlation between FT(4) and OH-BDEs was the most significant.
十溴二苯醚（BDE-209）是一种常用阻燃剂的主要成分，最近的研究表明其可被生物代谢。本研究采用腹腔注射的方式，将 BDE-209 以 50 至 1000ng/g 湿重的五个名义梯度浓度单次暴露于幼虹鳟（Oncorhynchus mykiss）21 天，然后采集肝脏、肾脏和血液样本，分析其脱溴、羟基化和甲氧基化代谢物。评估了不同组织中 BDE-209 代谢物水平与血浆中甲状腺激素（TH）水平之间的关系。结果表明，BDE-209 可代谢为脱溴 BDEs、甲氧基 BDEs（MeO-BDEs）和羟基 BDEs（OH-BDEs）。这三种代谢物的水平具有组织依赖性。血浆中的这些 TH 水平，包括总甲状腺素（TT(4)）、游离甲状腺素（FT(4)）、总三碘甲状腺原氨酸（TT(3)）和游离三碘甲状腺原氨酸（FT(3)），均受到 BDE-209 代谢的显著影响。然而，只有 FT(4)水平与 MeO-BDE 和 OH-BDE 代谢物呈负相关，其中 FT(4)与 OH-BDEs 的相关性最为显著。
