Microbiology and Immunology Section, Department of Animal Health, University of León, 24007 León, Spain.
Cell Immunol. 2012 May-Jun;277(1-2):74-82. doi: 10.1016/j.cellimm.2012.05.010. Epub 2012 May 23.
Four groups of colostrum-deprived pigs were immunized with Porcilis Glässer® (PG) or with subunit vaccines developed by us (rTbpA, NPAPT(M) or NPAPT(Cp)) against Glässer's disease, and they were challenged with 3×10(8)CFU of Haemophilus parasuis. A strong reduction in CD3(+)γδTCR(+) cells was seen in non-immunized control and scarcely protected (rTbpA) groups, suggesting that these cells could represent a target of H. parasuis infection. A significant increase in CD172α(+)CD163(+) cells was detected in all groups but PG, while a reduction in SLAIIDR(+) molecules expression was observed after challenge in control animals. Significant increases in CD3ε(+)CD8α(+)CD8β(+) and B cells were detected respectively in control and NPAPT groups, and in scarcely (rTbpA) and well-protected (NPAPT(M) and NPAPT(Cp)) groups. Finally, a greater response in CD4(+)CD8α(-) cells was observed in NPAPT(Cp) compared to NPAPT(M) and PG groups. These results state the potential of NPAPT antigen for developing effective vaccines against Glässer's disease.
四组初乳缺乏的猪用 Porcilis Glässer®（PG）或我们开发的亚单位疫苗（rTbpA、NPAPT（M）或 NPAPT（Cp））进行了针对格拉泽氏病的免疫接种，并接受了 3×10（8）CFU 的副猪嗜血杆菌的攻毒。未免疫对照和几乎未保护（rTbpA）组中观察到 CD3（+）γδ TCR（+）细胞的强烈减少，表明这些细胞可能是副猪嗜血杆菌感染的靶标。所有组均检测到 CD172α（+）CD163（+）细胞的显著增加，但 PG 组除外，而在对照动物攻毒后观察到 SLAIIDR（+）分子表达减少。在对照组和 NPAPT 组中分别检测到 CD3ε（+）CD8α（+）CD8β（+）和 B 细胞的显著增加，而在几乎未保护（rTbpA）和良好保护（NPAPT（M）和 NPAPT（Cp））组中也检测到显著增加。最后，与 NPAPT（M）和 PG 组相比，NPAPT（Cp）组中 CD4（+）CD8α（-）细胞的反应更大。这些结果表明 NPAPT 抗原具有开发针对格拉泽氏病的有效疫苗的潜力。
