Center of Clinical Laboratory, Zhongshan Hospital, Medical College of Xiamen University, Xiamen 361004, China.
Int Immunopharmacol. 2012 Sep;14(1):94-8. doi: 10.1016/j.intimp.2012.06.008. Epub 2012 Jun 18.
The most frequent symptoms among the manifestations of cow milk allergy (CMA) are gastrointestinal. CMA pathogenesis involves immunological mechanisms with participation of immunocompetent cells, production of immunoglobulin E (IgE) and immunoglobulin G (IgG). We aim to determine whether cow milk-specific IgE antibodies coexist with cow milk-specific IgG antibodies in CMA patients with diarrhea symptom, and if there is any relationship between both antibody types. 65 CMA patients (average age of 17 years, ranging from 2 to 74 years), all of who had diarrhea symptom of CMA, were enrolled in this study. The total cow IgE and IgG subclass in serum were measured by electrochemiluminescence immunoassay and rate immune scatter turbidimetry, respectively. And also the cow milk-specific IgE was determined by enzyme-linked immunosorbent assay. The number of eosinophils in serum was calculated by Sysmex XE-2100 Hematology Analyzer. Our data showed that both cow milk-specific IgG and IgE levels were significantly elevated in CMA patients compared to those of age-matched control subjects. Out of the 65 CMA patients, 40 showed elevated cow milk-specific IgE antibody level, among which, 28 cases presented highly sensitive reaction to cow milk-specific IgG, along with each six of moderate and mild sensitive reaction to cow milk-specific IgG; while 20 showed elevated total IgG levels. The IgG3 positive rate was 16.9%, which was the highest. A moderate correlation between cow milk-specific IgE and cow milk-specific IgG was found in the CMA patients (r=0.415, P=0.001). The results indicated that cow milk-specific IgE antibodies could coexist with cow milk-specific IgG antibodies in patients suffering from CMA. The aberrant changes in the concentration of cow milk-specific IgE antibodies were associated with cow milk-specific IgG antibodies.
牛奶过敏（CMA）的最常见症状是胃肠道。CMA 的发病机制涉及免疫机制，包括免疫活性细胞的参与、免疫球蛋白 E（IgE）和免疫球蛋白 G（IgG）的产生。我们旨在确定患有腹泻症状的 CMA 患者中是否存在牛奶特异性 IgE 抗体与牛奶特异性 IgG 抗体共存的情况，以及这两种抗体类型之间是否存在任何关系。
本研究纳入了 65 例 CMA 患者（平均年龄 17 岁，年龄范围为 2-74 岁），所有患者均有 CMA 腹泻症状。通过电化学发光免疫分析法和速率免疫散射比浊法分别检测血清总 IgE 和 IgG 亚类，通过酶联免疫吸附法测定牛奶特异性 IgE。通过 Sysmex XE-2100 血液分析仪计算血清嗜酸性粒细胞数。
我们的数据显示，与年龄匹配的对照组相比，CMA 患者的牛奶特异性 IgG 和 IgE 水平均显著升高。在 65 例 CMA 患者中，40 例牛奶特异性 IgE 抗体水平升高，其中 28 例对牛奶特异性 IgG 呈高度敏感反应，伴有 6 例中度和轻度敏感反应；而 20 例总 IgG 水平升高。IgG3 阳性率为 16.9%，最高。在 CMA 患者中发现牛奶特异性 IgE 与牛奶特异性 IgG 之间存在中度相关性（r=0.415，P=0.001）。
结果表明，CMA 患者中存在牛奶特异性 IgE 抗体与牛奶特异性 IgG 抗体共存的情况。牛奶特异性 IgE 抗体浓度的异常变化与牛奶特异性 IgG 抗体有关。
