Slocum H K, Malmberg M, Greco W R, Parsons J C, Rustum Y M
Department of Experimental Therapeutics, Roswell Park Memorial Institute, Buffalo, New York 14263.
Cytometry. 1990;11(7):793-804. doi: 10.1002/cyto.990110705.
This paper describes the evaluation of a colony formation assay using automated image analysis, which permits the tracking of growth at the individual colony level, such that a growth rate can be estimated for each colony followed. In principle, this will permit quantitative characterization of cellular heterogeneity in growth rate and cellular heterogeneity in response to proliferation-modifying agents. In addition, we have demonstrated the possibility of using correlative microscopy to relate growth rate to other parameters, using metabolic viability as an example. This should be useful for determining cellular characteristics associated with proliferative behavior and response to proliferation-modifying agents.
本文描述了使用自动图像分析对集落形成试验进行的评估，该分析允许在单个集落水平上追踪生长情况，从而能够为每个追踪的集落估计生长速率。原则上，这将允许对生长速率的细胞异质性以及对增殖调节剂的反应中的细胞异质性进行定量表征。此外，我们已经证明了使用相关显微镜将生长速率与其他参数相关联的可能性，以代谢活力为例。这对于确定与增殖行为和对增殖调节剂的反应相关的细胞特征应该是有用的。
