Robertson F M, Gilmour S K, Beavis A J, O'Connell S M, Conney A H, Huang M T, Laskin J D, Hietala O A, O'Brien T G
Department of Surgery, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, New Brunswick 08903.
Cytometry. 1990;11(7):832-6. doi: 10.1002/cyto.990110709.
Using flow cytometry in combination with membrane permeabilization techniques to enhance binding of antibodies with immunoreactive protein within the cytoplasm, we have developed a method to examine the ornithine decarboxylase (ODC) activity present within subpopulations of epidermal cells following acute and chronic exposure to the phorbol ester tumor promoter 12-0-tetradecanoylphorbol-13-acetate (TPA). The method described has the sensitivity to detect basal levels of ODC as well as increases in ODC at early time points following treatment with TPA and has the additional advantage of allowing subpopulation identification and characterization.
我们运用流式细胞术并结合细胞膜通透化技术，以增强抗体与细胞质内免疫反应性蛋白的结合，由此开发出一种方法，用于检测在急性和慢性暴露于佛波酯肿瘤启动子12-0-十四酰佛波醇-13-乙酸酯（TPA）后，表皮细胞亚群中存在的鸟氨酸脱羧酶（ODC）活性。所描述的方法具有检测ODC基础水平的灵敏度，以及在TPA处理后早期时间点ODC增加的检测能力，并且还具有允许亚群鉴定和表征的额外优势。
