von Melchner H, Ruley H E
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139.
Environ Health Perspect. 1990 Aug;88:141-8. doi: 10.1289/ehp.9088141.
By exploiting the ability of retroviruses to move genes into random sites of mammalian genomes and by exploiting some features of their replication, retrovirus vectors have been developed that select for instances in which the virus integrates into expressed genes. Since integrated proviruses tag transcriptionally active sites, the vectors provide a means to identify and isolate promoters active in different cell types. Furthermore, the viruses may be useful as insertional mutagens, since they select for instances in which integration occurs into expressed sites. This reduces the number of integrants needed to screen for loss of gene function and may enable genes controlling phenotypes in mammalian cells to be isolated.
通过利用逆转录病毒将基因转移到哺乳动物基因组随机位点的能力，并利用其复制的一些特性，已开发出逆转录病毒载体，用于筛选病毒整合到表达基因中的情况。由于整合的原病毒标记转录活性位点，这些载体提供了一种识别和分离在不同细胞类型中活跃的启动子的方法。此外，这些病毒可用作插入诱变剂，因为它们筛选整合到表达位点的情况。这减少了筛选基因功能丧失所需的整合体数量，并可能使控制哺乳动物细胞表型的基因得以分离。
