通过噬菌体展示技术筛选人源Fab文库。
Selection of human Fab libraries by phage display.
作者信息
Rader Christoph
机构信息
Experimental Transplantation and Immunology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
出版信息
Methods Mol Biol. 2012;901:81-99. doi: 10.1007/978-1-61779-931-0_5.
PMID:22723095
Abstract
This protocol describes the selection of human antibody libraries in Fab format by phage display. It includes panning against immobilized antigens, biotinylated antigens in solution, and cell surface antigens.
摘要
本方案描述了通过噬菌体展示选择Fab格式的人抗体文库的方法。它包括针对固定化抗原、溶液中的生物素化抗原以及细胞表面抗原进行淘选。
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