Dupont H, Cayrol C, Deparis P
Centre de Biologie du Développement (CNRS UA 675), Université Paul Sabatier, Toulouse, France.
Gen Comp Endocrinol. 1990 Sep;79(3):477-82. doi: 10.1016/0016-6480(90)90078-z.
Previous investigations of the liver estrogen-specific binding in the newt, Pleurodeles waltl, have identified in the cytosol fraction, only from normal males, a new middle-affinity estrogen-binding component (MEBC) displaying the properties of type II sites reported in various tissues of vertebrates. The present work demonstrates that MEBC sites are not unique to the male but are also present in the nuclei of female animals. However, comparative study between males and females of liver nuclear sites under various extraction conditions shows sex-linked differences in the subnuclear localization. The relationships between the association states of MEBC in the nuclear compartment according to sex and their presence or absence in the cytosol fraction are discussed.
以往对火蝾螈（Pleurodeles waltl）肝脏雌激素特异性结合的研究发现，仅在正常雄性动物的胞质溶胶部分中，有一种新的中等亲和力雌激素结合成分（MEBC），它具有在脊椎动物各种组织中报道的II型位点的特性。目前的研究表明，MEBC位点并非雄性所特有，在雌性动物的细胞核中也存在。然而，在不同提取条件下对肝脏细胞核位点的雄性和雌性进行的比较研究表明，亚核定位存在性别关联差异。本文讨论了根据性别，MEBC在核区室中的结合状态与其在胞质溶胶部分中是否存在之间的关系。
