Institute of Agriculture and Life Sciences, and ‡Division of Applied Life Science (BK21 Program), Gyeongsang National University , Jinju, Gyeongnam 660-701, Republic of Korea.
J Agric Food Chem. 2012 Aug 1;60(30):7392-7. doi: 10.1021/jf302484m. Epub 2012 Jul 17.
Attraction of the Bursaphelenchus xylophilus nematode toward 18 volatiles of Pinus species was evaluated by a Petri-dish bioassay under laboratory conditions to develop a rapid diagnostic kit. Among these compounds, α-pinene, β-pinene, and camphor showed significantly higher attractiveness to B. xylophilus in both the reproductive and dispersal stages, whereas these compounds were not active against Bursaphelenchus mucronatus . A trap tube was developed as a diagnostic kit, which consisted of a tube filled with 0.8% agar and a matrix impregnated with an attractant: α-pinene, β-pinene, or camphor. All tested compounds attracted a significantly higher number of B. xylophilus than that in the control treatment. No significant difference was observed among attractants. The cotton-ball matrix was significantly more effective than the filter-paper matrix for attracting B. xylophilus in the artificial pupal chamber bioassay. In a bioassay with pine wood nematode (PWN)-infected pine tree logs, B. xylophilus was initially attracted after an 8 h trap period and the number of B. xylophilus increased with time. The trap tube using camphor and the cotton-ball matrix were most effective for attracting B. xylophilus . The semiochemical-based tube-trapping method is simple to use, requires minimal labor, and is economical and effective for detecting B. xylophilus living in host pine trees during field sampling.
在实验室条件下，通过培养皿生物测定法评估了松材线虫对 18 种松树挥发物的吸引力，以开发一种快速诊断试剂盒。在这些化合物中，α-蒎烯、β-蒎烯和樟脑在生殖和扩散阶段对松材线虫表现出明显更高的吸引力，而这些化合物对柏肤小蠹无活性。开发了一种诱捕管作为诊断试剂盒，该管由填充有 0.8%琼脂的管和浸渍有引诱剂的基质组成：α-蒎烯、β-蒎烯或樟脑。所有测试的化合物都比对照处理吸引了明显更多的松材线虫。引诱剂之间没有观察到显著差异。在人工蛹室生物测定中，棉花球基质比滤纸基质更有效地吸引松材线虫。在感染松材线虫的松树原木生物测定中，松材线虫在 8 小时的诱捕期后最初被吸引，并且随着时间的推移，松材线虫的数量增加。使用樟脑和棉花球基质的诱捕管对吸引松材线虫最有效。基于半化学物质的管诱捕方法简单易用，所需劳动力最少，在田间采样时用于检测寄生于宿主松树上的松材线虫既经济又有效。
