Institute of Biochemistry and Molecular Biology, School of Life Sciences, Lanzhou University, 222 Tianshui South Road, Lanzhou, Gansu 730000, China.
Brain Res Bull. 2012 Sep 1;88(6):574-80. doi: 10.1016/j.brainresbull.2012.06.003. Epub 2012 Jun 21.
Apelin, a novel bioactive peptide highly concentrated in the brain, is identified as the endogenous ligand for angiotensin-like 1 receptor (APJ). The present study was designed to investigate the effect of apelin-13 on emotion-related behavior using the forced swimming test (FST) and tail suspension test (TST). Intracerebroventricular (i.c.v.) administration of apelin-13 (0.3, 1 and 3μg/mouse) dose-dependently increased the immobility time in the FST and TST, compared with control group. However, the APJ receptor antagonist apelin-13(F13A) (0.3-10μg/mouse, i.c.v.) had no influence on immobility time in the FST. The increase of immobility time induced by apelin-13 was significantly blocked by apelin-13(F13A), non-selective opioid receptor antagonist naloxone and κ-opioid receptor antagonist nor-binaltorphimine dihydrochloride (nor-BNI), respectively, but not the non-selective corticotrophin-releasing factor (CRF) receptor antagonist α-helical CRF(9-41) in the FST. In order to eliminate the possibility of a false-positive result in the FST or TST, spontaneous activity and motor function were checked. The results demonstrate that apelin-13 alone or antagonists co-administered with apelin-13 did influence spontaneous activity counts. And apelin-13 had no effect on the motor behavior in the rotarod test and wire hanging test. These results indicate that centrally administered apelin-13 elicited depression-like behavior in mice, which was mediated via APJ receptor and κ-opioid receptor, but not CRF receptor.
Apelin，一种高度集中在大脑中的新型生物活性肽，被鉴定为血管紧张素样 1 受体（APJ）的内源性配体。本研究旨在通过强迫游泳试验（FST）和悬尾试验（TST）研究 apelin-13 对情绪相关行为的影响。脑室内（i.c.v.）给予 apelin-13（0.3、1 和 3μg/只）剂量依赖性地增加 FST 和 TST 的不动时间，与对照组相比。然而，APJ 受体拮抗剂 apelin-13(F13A)（0.3-10μg/只，i.c.v.）对 FST 中的不动时间没有影响。Apelin-13 诱导的不动时间增加被 apelin-13(F13A)、非选择性阿片受体拮抗剂纳洛酮和κ-阿片受体拮抗剂 nor-binaltorphimine 二盐酸盐（nor-BNI）分别显著阻断，但 FST 中的非选择性促肾上腺皮质激素释放因子（CRF）受体拮抗剂α-螺旋 CRF(9-41)则不然。为了消除 FST 或 TST 中出现假阳性结果的可能性，检查了自发活动和运动功能。结果表明，apelin-13 单独或与拮抗剂共同给予 apelin-13 并不影响自发活动计数。并且 apelin-13 对旋转棒试验和悬线试验中的运动行为没有影响。这些结果表明，中枢给予 apelin-13 可引起小鼠抑郁样行为，这是通过 APJ 受体和κ-阿片受体介导的，而不是通过 CRF 受体介导的。
