Xiao Wen-De, Zhong Zhao-Ming, Tang Yong-Zhi, Xu Zi-Xing, Xu Zhun, Chen Jian-Ting
Department of Spine Surgery, Nanfang Hospital of Southern Medical University, Guangzhou, Guangdong Province, China.
Saudi Med J. 2012 Jun;33(6):601-7.
To generate a novel porous poly(D,L-lactide)/nacre nanocomposite hollow scaffold.
This study was performed in the Department of Spine Surgery, Southern Medical University, Guangzhou, China from September 2010 to September 2011. Nacre nanoparticles were prepared using a physical process and identified by x-ray diffraction and transmission electron microscopy, to generate a novel scaffold though the salt leaching processing technique. The morphology and structure properties of this scaffold were further investigated under scanning electron microscope and mechanical property testing. Additionally, the biological characteristics were evaluated by cell culture experiments in vitro. Thirty-six rabbits were randomly divided into 3 groups. The defects were implanted with/without poly(D,L-lactide)/nacre scaffold or poly(D,L-lactide) scaffold. The results were assessed by radiographs and bone mineral density to monitor bone repairing.
The nacre nanoparticles were spherical in shape, with a diameter range from 45-95 nm. The scaffolds possessed an interconnected porous structure with an average pore size of 322.5+/-50.8 μm, and exhibited a high porosity (82.5 +/-0.8%), as well as good compressive strength of 4.5+/-0.25 Mpa. Primary biocompatibility experiments in vitro showed that cells adhered and proliferated well on the scaffolds. The animal study further demonstrated that the scaffolds could repair the critical size segmental bone defects in 12 weeks.
Newly established scaffolds may serve as a promising biomaterial for bone tissue engineering.
制备一种新型的多孔聚（D，L-丙交酯）/珍珠层纳米复合中空支架。
本研究于2010年9月至2011年9月在中国广州南方医科大学脊柱外科进行。采用物理方法制备珍珠层纳米颗粒，并通过X射线衍射和透射电子显微镜进行鉴定，通过盐析处理技术制备新型支架。在扫描电子显微镜下进一步研究该支架的形态和结构特性，并进行力学性能测试。此外，通过体外细胞培养实验评估其生物学特性。36只兔子随机分为3组。在缺损处植入或不植入聚（D，L-丙交酯）/珍珠层支架或聚（D，L-丙交酯）支架。通过X线片和骨密度评估结果以监测骨修复情况。
珍珠层纳米颗粒呈球形，直径范围为45-95nm。支架具有相互连通的多孔结构，平均孔径为322.5±50.8μm，孔隙率高（82.5±0.8%），抗压强度良好，为4.5±0.25MPa。体外初步生物相容性实验表明细胞在支架上黏附及增殖良好。动物研究进一步证明该支架可在12周内修复临界尺寸的节段性骨缺损。
新制备的支架可能成为骨组织工程中有前景的生物材料。
