Knight M T, Wood D W, Black J F, Gosney G, Rigney R O, Agin J R, Gravens C K, Farnham S M
Q Laboratories, Inc., Cincinnati, OH 45214.
J Assoc Off Anal Chem. 1990 Sep-Oct;73(5):729-33.
Twelve laboratories evaluated the Gram-Negative Identification (GNI) Card to identify members of the Enterobacteriaceae. Eighty-four isolates, previously isolated from foods, were used in the collaborative study; the isolates represented 12 genera within the Enterobacteriaceae group. Each collaborator streaked each isolate on tryptic soy agar plates for purity. In the method, plates are incubated 18-24 h at 35 degrees C. Isolated colonies are then subcultured to tryptic soy agar slants and incubated 18-24 h at 35 degrees C. An emulsion is made from the growth on the slant in 1.8 mL 0.45% sodium chloride solution. The GNI Card is filled and placed in a reader/incubator. Isolates are identified and an identification is printed. The Vitek System correctly identified 96.7% of Salmonella sp., 97.0% of Escherichia coli, and an average of 93.8% of the other enteric genera. The method using the Vitek System and GNI Card has been approved interim official first action by AOAC as a screening method for the presumptive identification of Salmonella sp., E. coli, and other Enterobacteriaceae isolated from foods.
十二个实验室对革兰氏阴性菌鉴定（GNI）卡进行了评估，以鉴定肠杆菌科的成员。协作研究中使用了84株先前从食品中分离出的菌株；这些菌株代表了肠杆菌科内的12个属。每位合作者将每种菌株划线接种在胰蛋白胨大豆琼脂平板上以确保纯度。在该方法中，平板在35℃下孵育18 - 24小时。然后将分离出的菌落转接至胰蛋白胨大豆琼脂斜面上，并在35℃下孵育18 - 24小时。用1.8 mL 0.45%氯化钠溶液将斜面上的生长物制成菌悬液。将GNI卡填充好并放入读数器/培养箱中。对菌株进行鉴定并打印出鉴定结果。Vitek系统正确鉴定出96.7%的沙门氏菌属、97.0%的大肠杆菌以及其他肠道菌属平均93.8%。使用Vitek系统和GNI卡的方法已被AOAC批准为临时官方首次行动方法，作为从食品中分离出的沙门氏菌属、大肠杆菌和其他肠杆菌科细菌的初步鉴定筛选方法。
