Harris L, Humber J
Kraft General Foods, Technology Center, Glenview, IL 60025.
J AOAC Int. 1993 Jul-Aug;76(4):822-30.
A collaborative study was conducted to evaluate the performance of the AutoMicrobic System Gram-Positive Identification (GPI) and Gram-Negative Identification (GNI) test kits to biochemically characterize Listeria spp. Thirteen laboratories each tested 97 food and environmental isolates, representing the 7 species of Listeria, as well as 11 additional genera of Gram-positive rods. Each collaborator inoculated both a GPI and a GNI card with a pure culture of each organism. The AutoMicrobic System identified the isolates and printed out the biochemical results. The GPI card is used to obtain a species identification and a mannitol reaction result, and the GNI card is used to obtain rhamnose and xylose reaction results. Organisms are classified into species groups and can be further distinguished on the basis of hemolysis or nitrate reduction tests. The AutoMicrobic System method correctly classified 90.8% of the Listeria spp. isolates and 100% of the non-Listeria isolates. The AutoMicrobic System method was adopted first action by AOAC International for the biochemical characterization of Listeria spp. isolated from food and environmental sources.
开展了一项合作研究，以评估自动微生物系统革兰氏阳性菌鉴定（GPI）和革兰氏阴性菌鉴定（GNI）测试试剂盒对李斯特菌属进行生化特征分析的性能。13个实验室分别对97株食品和环境分离株进行了检测，这些分离株代表了7种李斯特菌以及另外11个革兰氏阳性杆菌属。每位合作者用每种微生物的纯培养物接种一张GPI卡和一张GNI卡。自动微生物系统对分离株进行鉴定并打印出生化结果。GPI卡用于获得菌种鉴定结果和甘露醇反应结果，GNI卡用于获得鼠李糖和木糖反应结果。微生物被分类到不同的菌种组，并且可以根据溶血或硝酸盐还原试验进一步区分。自动微生物系统方法正确分类了90.8%的李斯特菌属分离株和100%的非李斯特菌分离株。自动微生物系统方法被美国官方分析化学师协会国际组织作为对从食品和环境来源分离出的李斯特菌属进行生化特征分析的首选方法。
