Department of Urology, University of Athens Medical School, Athens, Greece.
Andrologia. 2013 Apr;45(2):128-34. doi: 10.1111/j.1439-0272.2012.01324.x. Epub 2012 Jun 26.
Previous studies have shown that alpha1-adrenergic receptor antagonists may alter seminal vesicle contractility and impair fertility in male rats. This study was designed to investigate the effects of terazosin on the catalase expression in the seminal vesicles and the lipid peroxidation of the seminal fluid in normal adult rats. Wistar rats were treated with terazosin (1.2 mg kg(-1) body weight, given orally every second day) for 120 days. Catalase expression was assessed immunohistochemically in tissue sections of the seminal vesicles, and lipid peroxidation was estimated by measuring the malondialdehyde (MDA) levels in the seminal vesicles' fluid. The seminal vesicles in terazosin-treated rats were particularly distended in comparison with those of controls, and their secreting epithelium was suppressed. Cytoplasmic catalase expression in the secreting epithelial cells (% of cells) was increased in terazosin-treated specimens in comparison with controls (76.1 ± 17.1 versus 51.3 ± 25.1, P = 0.005). MDA levels (μm) were also higher in samples from treated subjects in comparison with controls (2.67 ± 1.19 versus 1.39 ± 0.19, P = 0.01). Although the direct effect of terazosin treatment on the seminal vesicles is that of impaired contractility, an indirect effect is that on fertility by increasing lipid peroxidation in the seminal fluid and/or through degrading of hydrogen peroxide that is essential for sperm capacitation.
先前的研究表明，α1-肾上腺素能受体拮抗剂可能会改变精囊的收缩性并损害雄性大鼠的生育能力。本研究旨在研究特拉唑嗪对正常成年大鼠精囊过氧化氢酶表达和精液脂质过氧化的影响。Wistar 大鼠每天口服给予特拉唑嗪（1.2mg/kg 体重，每两天一次）120 天。通过免疫组织化学评估精囊组织切片中的过氧化氢酶表达，并通过测量精囊液中的丙二醛（MDA）水平来估计脂质过氧化。与对照组相比，特拉唑嗪治疗组的大鼠精囊特别扩张，其分泌上皮受到抑制。与对照组相比（76.1±17.1%对 51.3±25.1%，P=0.005），治疗组分泌上皮细胞中的细胞质过氧化氢酶表达增加。与对照组相比（2.67±1.19μm 对 1.39±0.19μm，P=0.01），治疗组样本中的 MDA 水平也更高。尽管特拉唑嗪治疗对精囊的直接作用是收缩性受损，但间接作用是通过增加精液中的脂质过氧化和/或通过降解精子获能所必需的过氧化氢来降低生育能力。
