Department of Chemical Engineering, ‡Department of Mechanical Engineering, National Taiwan University, Taipei, Taiwan.
ACS Appl Mater Interfaces. 2012 Jul 25;4(7):3335-9. doi: 10.1021/am300918s. Epub 2012 Jun 28.
A new approach is presented to control cell attachment behavior on biocompatible substrates. Multiple layers of polylactic acid (PLA) were inkjet-printed on dry alginate films to create composite surfaces with rigidity variation. The printed films were submerged in cell culture medium and fibroblast 3T3-L1 cells were cultured on the printed films. 3T3-L1 cells were found to preferentially adhere on PLA surfaces with higher rigidity. The same approach was also used to create various cell attachment patterns. This study provides a new methodology to fabricate biodegradable matrix for favorable cell adhesion or patterning.
提出了一种新的方法来控制生物相容性基底上细胞的附着行为。将聚乳酸（PLA）的多层通过喷墨打印到干燥的藻酸盐薄膜上来制造具有刚度变化的复合表面。将打印的薄膜浸入细胞培养基中，并在打印的薄膜上培养成纤维细胞 3T3-L1。发现 3T3-L1 细胞优先附着在刚性较高的 PLA 表面上。同样的方法也被用来制造各种细胞附着模式。这项研究为制造有利于细胞附着或图案化的可生物降解基质提供了一种新方法。
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