Yu Guanhua, Fan Yubo
Department of Bioengineering, Beihang University, Beijing 100083, PR China.
J Biomater Sci Polym Ed. 2008;19(1):87-98. doi: 10.1163/156856208783227703.
A kind of novel natural polysaccharide (sodium alginate) porogen was developed to prepare a 3D biodegradable tissue-engineering scaffold. The sodium alginate particles were prepared by emulsification and subsequent ionic gelation. The size and morphology of the alginate particles was simply controlled by the stirring rate and the concentration of the cross-linking agent. ATR-FTIR spectroscopy demonstrated the existence of alginate molecules on the surface of the PLA scaffold. The water uptake of the scaffold made from alginate particles was obviously improved compared with the scaffold fabricated by KCl porogens. A MC3T3 osteoblast culture on the scaffolds showed that the alginate-modified PLA scaffolds significantly enhanced the osteoblast adhesion and proliferation. These results indicate that the alginate particle is a good porogen in the fabrication of 3D scaffolds for bone tissue engineering.
一种新型天然多糖（海藻酸钠）致孔剂被开发用于制备三维可生物降解组织工程支架。海藻酸钠颗粒通过乳化和随后的离子凝胶化制备。海藻酸钠颗粒的尺寸和形态可通过搅拌速率和交联剂浓度简单控制。衰减全反射傅里叶变换红外光谱（ATR-FTIR）表明聚乳酸（PLA）支架表面存在海藻酸钠分子。与由氯化钾致孔剂制备的支架相比，由海藻酸钠颗粒制成的支架的吸水率明显提高。在支架上进行的MC3T3成骨细胞培养表明，海藻酸钠改性的PLA支架显著增强了成骨细胞的粘附和增殖。这些结果表明，海藻酸钠颗粒是用于骨组织工程的三维支架制造中的良好致孔剂。
