Chen Shu-Juan, Lu Fang, Cheng Jia-An, Jiang Ming-Xing, Way Michael O
Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, 310058 Hangzhou, China.
Environ Entomol. 2012 Jun;41(3):469-77. doi: 10.1603/EN11195.
Wolbachia spp. are obligate intracellular bacteria present in reproductive tissues of many arthropod species. Wolbachia infection status and roles in host reproduction were studied in the rice water weevil, Lissorhoptrus oryzophilus Kuschel (Coleoptera, Curculionidae), an introduced species in China. We examined Wolbachia infection status in five populations in China where it reproduces parthenogenetically, and one native population in Southeast Texas, where it reproduces bisexually. All populations were infected by Wolbachia, and all specimens in each population were infected by Wolbachia of a single strain. Phylogenetic analyses based on multilocus sequence typing system indicated that Wolbachia in non-native L. oryzophilus weevils diverges evidently from those in native weevils. After treatments with tetracycline, parthenogenetic weevils reduced the fecundity significantly and eggs were not viable. Our results suggest that Wolbachia are necessary for oocyte production in L oryzophilus.
沃尔巴克氏体属是存在于许多节肢动物物种生殖组织中的专性细胞内细菌。在中国，稻水象甲（Lissorhoptrus oryzophilus Kuschel，鞘翅目，象甲科）是一种外来物种，我们对其体内的沃尔巴克氏体感染状况及其在宿主繁殖中的作用进行了研究。我们检测了中国五个孤雌生殖种群以及德克萨斯州东南部一个两性生殖本地种群的沃尔巴克氏体感染状况。所有种群均感染了沃尔巴克氏体，且每个种群中的所有标本均感染了单一菌株的沃尔巴克氏体。基于多位点序列分型系统的系统发育分析表明，非本地稻水象甲体内的沃尔巴克氏体与本地象甲体内的沃尔巴克氏体明显不同。用四环素处理后，孤雌生殖的象甲繁殖力显著降低，且所产的卵无法存活。我们的结果表明，沃尔巴克氏体对于稻水象甲的卵母细胞产生是必需的。
