Chen Haoliang, Zhang Hongyu, Zhu Kun Yan, Throne James E
State Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China.
Environ Entomol. 2012 Jun;41(3):697-705. doi: 10.1603/EN11311.
Development of the parasitoid Habrobracon hebetor (Say) (Hymenoptera: Braconidae) at low temperatures was determined to identify rearing conditions that might result in adults that were in reproductive diapause. Diapausing adults would be expected to survive cold storage longer than nondiapausing adults for use in biological control programs. Only a few eggs were found in the ovaries when H. hebetor females were reared during the immature stages at 17.5 and 20°C with a 16-h photoperiod, and the ovaries were poorly developed and contained no eggs when females were reared with a 10-h photoperiod in these low temperatures. Rearing H. hebetor at 17.5 and 20°C did not result in diapause of immature stages, but did appear to result in possible adult reproductive diapause when the immature stages were reared with a 10-h photoperiod. Females reared during the immature stages at 17.5°C with a10-h photoperiod lived longer and took longer to lay their first eggs and to lay 50% of their eggs than those females reared at 17.5°C with a16-h photoperiod. Females reared during the immature stages at 20°C with a10-h photoperiod took longer to lay their first eggs and to lay 50% of their eggs, and they had a lower respiration rate, than those females reared at 20°C with a16-h photoperiod. Females that were reared in conditions that appeared to induce reproductive diapause resumed oviposition and their respiration rate increased soon after being transferred to a higher temperature (27.5°C). Thus, females reared at a 10-h photoperiod at 17.5 and 20°C appear to enter reproductive diapause.
为确定可能导致成虫进入生殖滞育的饲养条件，对寄生蜂哈氏啮小蜂（Habrobracon hebetor (Say)，膜翅目：茧蜂科）在低温下的发育情况进行了研究。预计滞育成虫在冷藏条件下的存活时间会比非滞育成虫更长，以便用于生物防治计划。当哈氏啮小蜂雌虫在未成熟阶段于17.5℃和20℃、16小时光照周期下饲养时，卵巢中仅发现少数卵；而在这些低温条件下，当雌虫在10小时光照周期下饲养时，卵巢发育不良且无卵。在17.5℃和20℃饲养哈氏啮小蜂，未成熟阶段不会进入滞育，但当未成熟阶段在10小时光照周期下饲养时，似乎会导致成虫进入可能的生殖滞育。与在17.5℃、16小时光照周期下饲养的雌虫相比，在未成熟阶段于17.5℃、10小时光照周期下饲养的雌虫寿命更长，产下第一枚卵以及产下50%的卵所需时间更长。与在20℃、16小时光照周期下饲养的雌虫相比，在未成熟阶段于20℃、10小时光照周期下饲养的雌虫产下第一枚卵以及产下50%的卵所需时间更长，且呼吸速率更低。在似乎诱导生殖滞育的条件下饲养的雌虫，在转移到较高温度（27.5℃）后不久便恢复产卵，呼吸速率也增加。因此，在17.5℃和20℃、10小时光照周期下饲养的雌虫似乎进入了生殖滞育。
