ABF Analytisch-Biologisches Forschungslabor GmbH, Munich, Germany.
J Sep Sci. 2012 Jun;35(10-11):1227-35. doi: 10.1002/jssc.201200056.
Eicosanoids are potent lipid mediators involved in numerous physiological and pathophysiological processes. Precursors are polyunsaturated fatty acids liberated from membrane phospholipids. Thus, profiling and quantification of these molecules has gained a lot of attention during last years. Eicosanoids and phospholipids are commonly profiled by LC-MS/MSbecause this technique allows accurate quantification within acceptable run-times. This article therefore focuses on liquid chromatography and the ESI-MS/MS analysis of proinflammatory lipid mediators, particularly arachidonic acid (C20:4) derived eicosanoids and their precursors phospholipids. Recent analytical developments for quantification of these compounds are highlighted and analytical challenges are discussed. Furthermore, applications such as the use of these molecules as biomarkers are presented.
类二十烷酸是参与多种生理和病理生理过程的强效脂质介质。前体是从膜磷脂中释放的多不饱和脂肪酸。因此，近年来，这些分子的分析和定量受到了广泛关注。类二十烷酸和磷脂通常通过 LC-MS/MS 进行分析，因为该技术可以在可接受的运行时间内进行准确的定量。本文因此重点介绍了用于分析促炎脂质介质（特别是花生四烯酸（C20：4）衍生的类二十烷酸及其前体磷脂）的液相色谱和 ESI-MS/MS 分析。重点介绍了这些化合物定量的最新分析进展，并讨论了分析挑战。此外，还介绍了这些分子作为生物标志物的应用。
