Department of Thoracic Surgery, School of Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey.
Eur J Cardiothorac Surg. 2013 Jan;43(1):163-7. doi: 10.1093/ejcts/ezs313. Epub 2012 Jun 24.
In our study, we aimed to investigate the anti-inflammatory mediator effects of budesonide (BS), an inhaled corticosteroid and interleukin-10 (IL-10) on a pulmonary contusion in an experimental rat model in which an isolated bilateral pulmonary contusion was created by blunt thoracic trauma.
Fifty-five male Sprague-Dawley rats were used in the study. Sham, control, BS and IL-10 groups were created. A pulmonary contusion was created by performing isolated blunt thoracic trauma in all groups except for the sham group. The trauma's severity was determined as 1.45 J. BS and IL-10 were administered orogastrically to the respective groups 30 min before trauma, and orogastrically and intraperitoneally, respectively, on the first and second days after the trauma. Only the blunt thoracic trauma was performed for the control group. SatO(2), PaO(2) and PaCO(2), blood glutathione, malondialdehyde (MDA) and tumour necrosis factor-α (TNFα) values were recorded on the zeroth, first, second and third days. The histopathological examination and the bronchoalveolar lavage cell count were performed on pulmonary tissues.
Blood gas analysis revealed that SatO(2) and PaO(2) values on the first and second days were significantly lower in the control, BS and IL-10 groups compared with the sham group (P < 0.05). The SatO(2) and PaO(2) values on the third day in the BS and IL-10 groups were higher than in the control group (P < 0.05). The mean MDA in the control group was higher than in the sham, BS and IL-10 groups (P < 0.05). The mean TNFα in the control group was higher than in the sham, BS and IL-10 groups (P < 0.05). Pulmonary pathology scoring in the control group was observed to be higher than in the sham, BS and IL-10 groups (P < 0.05).
In this rat experiment model in which an isolated pulmonary contusion was created by blunt trauma, BS and IL-10 were observed to reduce contusion severity in the lung and minimize the inflammatory reaction.
在我们的研究中，我们旨在通过钝性胸部创伤在实验大鼠模型中创建孤立的双侧肺挫伤，来研究布地奈德（BS）（一种吸入性皮质类固醇和白细胞介素 10（IL-10）对肺挫伤的抗炎介质作用。
本研究共使用了 55 只雄性 Sprague-Dawley 大鼠。建立了假手术、对照组、BS 组和 IL-10 组。除假手术组外，所有组均通过孤立的钝性胸部创伤来创建肺挫伤。创伤的严重程度确定为 1.45J。BS 和 IL-10 分别在创伤前 30 分钟，以及创伤后第 1 天和第 2 天通过口服和腹腔内给予相应的组。对照组仅进行钝性胸部创伤。在第 0、1、2 和第 3 天记录血氧饱和度（SatO2）、动脉血氧分压（PaO2）和动脉血二氧化碳分压（PaCO2）、血液谷胱甘肽、丙二醛（MDA）和肿瘤坏死因子-α（TNFα）值。对肺组织进行组织病理学检查和支气管肺泡灌洗细胞计数。
血气分析显示，与假手术组相比，对照组、BS 组和 IL-10 组在第 1 天和第 2 天的 SatO2 和 PaO2 值显著降低（P<0.05）。BS 组和 IL-10 组在第 3 天的 SatO2 和 PaO2 值高于对照组（P<0.05）。对照组的平均 MDA 值高于假手术组、BS 组和 IL-10 组（P<0.05）。对照组的平均 TNFα 值高于假手术组、BS 组和 IL-10 组（P<0.05）。对照组的肺病理学评分高于假手术组、BS 组和 IL-10 组（P<0.05）。
在通过钝性创伤创建孤立性肺挫伤的这个大鼠实验模型中，BS 和 IL-10 被观察到减轻了肺部挫伤的严重程度并最小化了炎症反应。
