Kuai Le, Chen Hao, Zhang Ting-Ting, Yang Hua-Yuan
College of Acupuncture-Moxibustion and Massage, Shanghai University of TCM, Shanghai 201203, China.
Zhongguo Zhen Jiu. 2012 Apr;32(4):331-7.
To observe the dose-effect relationship of electroacupuncture of different current intensities combined with Morphine of different dosage on alleviating the rats' tibial cancer pain, and explore the possible mechanism, which could provide the experiment basis for alleviating the tibial cancer pain by electroacupuncture combined with Morphine.
One hundred female Wistar rats were randomly divided into a normal group, a model group and eight treatment groups, 10 cases in each group. The rats in the treatment groups were treated by combined therapies of electroacupuncture of different intensities with 2 Hz /100 Hz dense-disperse wave on "Jiaji"(EX-B 2)and different dosage Morphine in 2 factor 3 level conditions, once a day for 6 days. The pain thresholds were observed before the treatment and 0 min, 1 h, 2 h and 5 h after the first treatment as well as after 3 and 6 times of treatments. The glial fibrillary acidic protein (GFAP) expression was determined by immunohistochemical method.
The rats' pain thresholds were significantly increased with electroacupuncture of 2 mA and 1 mA (all P < 0.01) on the 0 min, 1 h and 2 h of the first treatment, between which there were no significant differences (all P > 0.05). The pain threshold was still increased by electroacupuncture of 2 mA on the 5 h of the treatment (P < 0.01), while that of 1 mA failed to take effect (P > 0.05). After 3 and 6 times of treatments, both electroacupuncture of 2 mA and 1 mA had the effect of increasing the pain threshold (all P < 0.01), and the effect of 2 mA was superior to that of 1 mA (P < 0.05), had the synergistic effect with 5 mg/(kg x d) Morphine (P < 0.05). After 6 times of treatments, both electroacupuncture of 2 mA and 1 mA could inhibit the expression of GFAP (both P < 0.01), and there was no significant difference between them (P > 0.05). Both of 5 mg/(kg x d) and 2.5 mg/(kg x d) of Morphine, however, didn't bring about inhibition effect (P > 0.05).
There is a does-effect relationship on electroacupuncture of different current intensity for alleviating the tibial cancer pain in rats. The electroacupuncture with 2 mA, which is better than that with 1 mA, has the synergistic effect with 5 mg/(kg x d) of Morphine. The electroacupuncture can inhibit the expression of GFAP to cooperate with Morphine for the purpose of alleviating the rats' tibial cancer pain.
观察不同电流强度电针结合不同剂量吗啡对缓解大鼠胫骨癌痛的量效关系，并探讨其可能机制，为电针结合吗啡缓解胫骨癌痛提供实验依据。
将100只雌性Wistar大鼠随机分为正常组、模型组和8个治疗组，每组10只。治疗组大鼠在“夹脊”（EX - B 2）穴采用2 Hz /100 Hz疏密波、不同强度电针与不同剂量吗啡的二因素三水平联合治疗，每日1次，共6天。分别于治疗前及首次治疗后0 min、1 h、2 h、5 h以及治疗3次和6次后观察痛阈。采用免疫组化法检测胶质纤维酸性蛋白（GFAP）表达。
首次治疗0 min、1 h、2 h时，2 mA和1 mA电针均能显著提高大鼠痛阈（均P < 0.01），二者之间差异无统计学意义（均P > 0.05）。治疗5 h时，2 mA电针仍能提高痛阈（P < 0.01），而1 mA电针未起效（P > 0.05）。治疗3次和6次后，2 mA和1 mA电针均有提高痛阈的作用（均P < 0.01），且2 mA效果优于1 mA（P < 0.05），与5 mg/(kg·d)吗啡有协同作用（P < 0.05）。治疗6次后，2 mA和1 mA电针均能抑制GFAP表达（均P < 0.01），二者之间差异无统计学意义（P > 0.05）。而5 mg/(kg·d)和2.5 mg/(kg·d)吗啡均未产生抑制作用（P > 0.05）。
不同电流强度电针对缓解大鼠胫骨癌痛存在量效关系。2 mA电针效果优于1 mA，与5 mg/(kg·d)吗啡有协同作用。电针可抑制GFAP表达，与吗啡协同缓解大鼠胫骨癌痛。
