Department of Horticultural Science, Plants for Human Health Institute, North Carolina State University, 600 Laureate Way, Suite 1329, Kannapolis, North Carolina 28081, USA.
J Agric Food Chem. 2012 Jul 25;60(29):7238-44. doi: 10.1021/jf302475d. Epub 2012 Jul 12.
Brassica oleracea vegetables, such as broccoli (B. oleracea L. var. italica) and cauliflower (B. oleracea L. var. botrytis), are known to contain bioactive compounds associated with health, including three classes of photosynthetic lipid-soluble compounds: carotenoids, chlorophylls, and tocopherols. Carotenoids and chlorophylls are photosynthetic pigments. Tocopherols have vitamin E activity. Due to genetic and environmental variables, the amounts present in vegetables are not constant. To aid breeders in the development of Brassica cultivars with higher provitamin A and vitamin E contents and antioxidant activity, a more efficient method was developed to quantitate carotenoids, chlorophylls, and tocopherols in the edible portions of broccoli and cauliflower. The novel UPLC method separated five carotenoids, two chlorophylls, and two tocopherols in a single 30 min run, reducing the run time by half compared to previously published protocols. The objective of the study was to develop a faster, more effective extraction and quantitation methodology to screen large populations of Brassica germplasm, thus aiding breeders in producing superior vegetables with enhanced phytonutrient profiles.
甘蓝类蔬菜，如西兰花（B. oleracea L. var. italica）和菜花（B. oleracea L. var. botrytis），已知含有与健康相关的生物活性化合物，包括三类光合脂溶性化合物：类胡萝卜素、叶绿素和生育酚。类胡萝卜素和叶绿素是光合作用色素。生育酚具有维生素 E 活性。由于遗传和环境变量，蔬菜中的含量并不恒定。为了帮助育种者开发出富含维生素 A 前体和维生素 E 以及具有抗氧化活性的甘蓝类品种，开发了一种更有效的方法来定量分析西兰花和菜花可食用部分的类胡萝卜素、叶绿素和生育酚。新的 UPLC 方法在单次 30 分钟的运行中分离了五种类胡萝卜素、两种叶绿素和两种生育酚，与以前发表的方法相比，运行时间缩短了一半。本研究的目的是开发一种更快、更有效的提取和定量方法来筛选大量的甘蓝类种质资源，从而帮助育种者生产具有增强的植物营养素谱的优质蔬菜。
